Liposomes can be used as drug carriers, biocompatible with human cells. However, efficient on-demand release of the liposome payload still remains a challenge. Also, sophisticated liposome formulations, such as those encapsulating one or more drugs together with a suitable compound to facilitate triggered release, are usually prepared in complex multistep batch procedures with low (e.g. milligram) production rates. The aim of this work is to develop a continuous supercritical CO2 based scalable process for the production of near-infrared (NIR) light-sensitive liposomes. Hollow Gold Nanospheres (HGN), about 5 nm large, have been synthesized as light sensitive agents for triggered release and, then, they were embedded in controlled size liposomes (from 0.75 ± 0.19 μm to 1.76 ± 0.71 μm) together with biological active compounds Polyinosinic-polycytidylic Acid (PIC) and Bovine Serum Albumin (BSA), using a supercritical assisted process. High encapsulation efficiency of all components, up to 95%, with a gram-scale production of 1 g/h of HGN loaded liposomes have been obtained. Drug release tests verified that NIR light could be efficiently used as a stimulus on these vectors to achieve drug release on-demand. Produced liposomes presented high biocompatibility.
Efficient gram-scale continuous production of near-infrared-sensitive liposomes for light-triggered delivery of polyinosinic-polycytidylic acid / Apicella, D.; Arruebo, M.; Campardelli, R.; Encabo Berzosa, M.; Trucillo, P.; Sebastian, V.; Santamaria, J.; Reverchon, E.. - In: CHEMICAL ENGINEERING AND PROCESSING. - ISSN 0255-2701. - 146:(2019), p. 107709. [10.1016/j.cep.2019.107709]
Efficient gram-scale continuous production of near-infrared-sensitive liposomes for light-triggered delivery of polyinosinic-polycytidylic acid
Trucillo P.;
2019
Abstract
Liposomes can be used as drug carriers, biocompatible with human cells. However, efficient on-demand release of the liposome payload still remains a challenge. Also, sophisticated liposome formulations, such as those encapsulating one or more drugs together with a suitable compound to facilitate triggered release, are usually prepared in complex multistep batch procedures with low (e.g. milligram) production rates. The aim of this work is to develop a continuous supercritical CO2 based scalable process for the production of near-infrared (NIR) light-sensitive liposomes. Hollow Gold Nanospheres (HGN), about 5 nm large, have been synthesized as light sensitive agents for triggered release and, then, they were embedded in controlled size liposomes (from 0.75 ± 0.19 μm to 1.76 ± 0.71 μm) together with biological active compounds Polyinosinic-polycytidylic Acid (PIC) and Bovine Serum Albumin (BSA), using a supercritical assisted process. High encapsulation efficiency of all components, up to 95%, with a gram-scale production of 1 g/h of HGN loaded liposomes have been obtained. Drug release tests verified that NIR light could be efficiently used as a stimulus on these vectors to achieve drug release on-demand. Produced liposomes presented high biocompatibility.File | Dimensione | Formato | |
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Descrizione: Efficient gram-scale continuous production of near-infrared-sensitive liposomes for light-triggered delivery of polyinosinic-polycytidylic acid
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