The major enzyme component of idunorate sulfatase from human placenta was purified 30 000-fold by a five-step procedure. Sucrose gradient centrifugation of the native enzyme gave a molecular weight estimate of 80 000 ± 10 000. Electrophoresis in sodium dodecyl sulfate of the enzyme reduced with mercaptoethanol showed a protein band of Mr 82 000. We suggest that the enzyme is composed of a single polypeptide chain of Mr 80 000-90 000. © 1985.
Iduronate sulfatase from human placenta / DI NATALE, P; Daniele, Aurora. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 839:3(1985), pp. 258-261. [10.1016/0304-4165(85)90006-6]
Iduronate sulfatase from human placenta
DANIELE, Aurora
1985
Abstract
The major enzyme component of idunorate sulfatase from human placenta was purified 30 000-fold by a five-step procedure. Sucrose gradient centrifugation of the native enzyme gave a molecular weight estimate of 80 000 ± 10 000. Electrophoresis in sodium dodecyl sulfate of the enzyme reduced with mercaptoethanol showed a protein band of Mr 82 000. We suggest that the enzyme is composed of a single polypeptide chain of Mr 80 000-90 000. © 1985.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.