The fields of application of functional proteomics are not limited to the study of protein-protein interactions; they also extend to those involving protein complexes that bind DNA or RNA. These interactions affect fundamental processes such as replication, transcription, and repair in the case of DNA, as well as transport, translation, splicing, and silencing in the case of RNA. Analytical or preparative experimental approaches, both in vivo and in vitro, have been developed to isolate and identify DNA/RNA binding proteins by exploiting the advantage of the affinity shown by these proteins toward a specific oligonucleotide sequence. The present review proposes an overview of the approaches most commonly employed in proteomics applications for the identification of nucleic acid-binding proteins, such as affinity purification (AP) protocols, EMSA, chromatin purification methods, and CRISPR-based chromatin affinity purification, which are generally associated with mass spectrometry methodologies for the unbiased protein identification.

Protein-DNA/RNA Interactions: An Overview of Investigation Methods in the -Omics Era / Cozzolino, F.; Iacobucci, I.; Monaco, V.; Monti, M.. - In: JOURNAL OF PROTEOME RESEARCH. - ISSN 1535-3893. - 20:6(2021), pp. 3018-3030. [10.1021/acs.jproteome.1c00074]

Protein-DNA/RNA Interactions: An Overview of Investigation Methods in the -Omics Era

Cozzolino F.
Primo
;
Iacobucci I.;Monaco V.;Monti M.
2021

Abstract

The fields of application of functional proteomics are not limited to the study of protein-protein interactions; they also extend to those involving protein complexes that bind DNA or RNA. These interactions affect fundamental processes such as replication, transcription, and repair in the case of DNA, as well as transport, translation, splicing, and silencing in the case of RNA. Analytical or preparative experimental approaches, both in vivo and in vitro, have been developed to isolate and identify DNA/RNA binding proteins by exploiting the advantage of the affinity shown by these proteins toward a specific oligonucleotide sequence. The present review proposes an overview of the approaches most commonly employed in proteomics applications for the identification of nucleic acid-binding proteins, such as affinity purification (AP) protocols, EMSA, chromatin purification methods, and CRISPR-based chromatin affinity purification, which are generally associated with mass spectrometry methodologies for the unbiased protein identification.
2021
Protein-DNA/RNA Interactions: An Overview of Investigation Methods in the -Omics Era / Cozzolino, F.; Iacobucci, I.; Monaco, V.; Monti, M.. - In: JOURNAL OF PROTEOME RESEARCH. - ISSN 1535-3893. - 20:6(2021), pp. 3018-3030. [10.1021/acs.jproteome.1c00074]
File in questo prodotto:
File Dimensione Formato  
J. Proteome Res_2021.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Dominio pubblico
Dimensione 2.98 MB
Formato Adobe PDF
2.98 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/866886
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 45
  • ???jsp.display-item.citation.isi??? 41
social impact