Non-typhoid Salmonella (NTS) represents one of the major causes of foodborne diseases, which are made worse by the increasing emergence of antibiotic resistance. Thus, NTS are a significant and common public health concern. The purpose of this study is to investigate whether selection for phage-resistance alters bacterial phenotype, making this approach suitable for candidate vaccine preparation. We therefore compared two strains of Salmonella enterica serovar Rissen: RR (the phage-resistant strain) and RW (the phage-sensitive strain) in order to investigate a potential cost associated with the bacterium virulence. We tested the ability of both RR and RW to infect phagocytic and non-phagocytic cell lines, the activity of virulence factors associated with the main Type-3 secretory system (T3SS), as well as the canonic inflammatory mediators. The mutant RR strain—compared to the wildtype RW strain—induced in the host a weaker innate immune response. We suggest that the mitigated inflammatory response very likely is due to structural modifications of the lipopolysaccharide (LPS). Our results indicate that phage-resistance might be exploited as a means for the development of LPS-based antibacterial vaccines.

Bacteriophage-resistant salmonella rissen: An in vitro mitigated inflammatory response / Capparelli, R.; Cuomo, P.; Papaianni, M.; Pagano, C.; Montone, A. M. I.; Ricciardelli, A.; Iannelli, D.. - In: VIRUSES. - ISSN 1999-4915. - 13:12(2021), p. 2468. [10.3390/v13122468]

Bacteriophage-resistant salmonella rissen: An in vitro mitigated inflammatory response

Capparelli R.
;
Cuomo P.;Papaianni M.;Pagano C.;Ricciardelli A.;Iannelli D.
2021

Abstract

Non-typhoid Salmonella (NTS) represents one of the major causes of foodborne diseases, which are made worse by the increasing emergence of antibiotic resistance. Thus, NTS are a significant and common public health concern. The purpose of this study is to investigate whether selection for phage-resistance alters bacterial phenotype, making this approach suitable for candidate vaccine preparation. We therefore compared two strains of Salmonella enterica serovar Rissen: RR (the phage-resistant strain) and RW (the phage-sensitive strain) in order to investigate a potential cost associated with the bacterium virulence. We tested the ability of both RR and RW to infect phagocytic and non-phagocytic cell lines, the activity of virulence factors associated with the main Type-3 secretory system (T3SS), as well as the canonic inflammatory mediators. The mutant RR strain—compared to the wildtype RW strain—induced in the host a weaker innate immune response. We suggest that the mitigated inflammatory response very likely is due to structural modifications of the lipopolysaccharide (LPS). Our results indicate that phage-resistance might be exploited as a means for the development of LPS-based antibacterial vaccines.
2021
Bacteriophage-resistant salmonella rissen: An in vitro mitigated inflammatory response / Capparelli, R.; Cuomo, P.; Papaianni, M.; Pagano, C.; Montone, A. M. I.; Ricciardelli, A.; Iannelli, D.. - In: VIRUSES. - ISSN 1999-4915. - 13:12(2021), p. 2468. [10.3390/v13122468]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/866280
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