The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.
Radiation quality and intra-chromosomal aberrations: Size matters / Cornforth, M. N.; Durante, M.. - In: MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS. - ISSN 1383-5718. - 836:(2018), pp. 28-35. [10.1016/j.mrgentox.2018.05.002]
Radiation quality and intra-chromosomal aberrations: Size matters
Durante M.
2018
Abstract
The shift from plant to mammalian cell models in radiation cytogenetics hastened the development of methods suitable for the analysis of chromosome-type aberrations. These included methods to detect interchanges that take place between different chromosomes (dicentrics and translocations), and intrachanges occurring within a given chromosome (rings, interstitial deletions and inversions). In this review we consider the relationship between chromosome-type interchanges and intrachanges in response to changes in ionization density (linear energy transfer; LET). In that context, we discuss advantages and disadvantages of more modern methods used to measure intrachanges, and the implications that their increased resolution of measurement may have on the inter-to-intrachange fraction (i.e., the F-ratio). We conclude that the premise of the F-ratio is supported by its biophysical assumptions, but its intended use as an LET-dependent measure of prior radiation exposure is hampered mainly by our inability to accurately assess, on a cell-by-cell basis, inversions and interstitial deletions whose small sizes are below the detection limits of conventional cytogenetic techniques.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.