The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.
Design of a microfluidic device for the phase-contrast tomography of flowing cells / Villone, M. M.; Santonastaso, E.; Memmolo, P.; Trotta, G.; Merola, F.; Maffettone, P. L.; Ferraro, P.. - 11786:(2021), p. 1. (Intervento presentato al convegno Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 tenutosi a deu nel 2021) [10.1117/12.2592327].
Design of a microfluidic device for the phase-contrast tomography of flowing cells
Villone M. M.
Primo
;Memmolo P.;Merola F.;Maffettone P. L.;Ferraro P.
2021
Abstract
The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
