In recent years, the scientific community has focused on the development of new antibiotics to address the difficulties linked to biofilm-forming microorganisms and drug-resistant infections. In this respect, synthetic antimicrobial peptides (AMPs) are particularly regarded for their therapeutic potential against a broad spectrum of pathogens. In this work, the antimicrobial and antibiofilm activities of the peptide WMR-K towards single and dual species cultures of Candida albicans and Klebsiella pneumoniae were investigated. We found minimum inhibitory concentration (MIC) values for WMR-K of 10 µM for K. pneumoniae and of 200 µM for C. albicans. Furthermore, sub-MIC concentrations of peptide showed an in vitro inhibition of biofilm formation of mono and polymicrobial systems and also a good biofilm eradication even if higher concentrations of it are needed. In order to provide additional evidence for the effect of the examined peptide, a study of changes in extracellular metabolites excreted and/or uptaken from the culture medium (metabolomic footprinting) in the poly-microbial association of C. albicans and K. pneumoniae in presence and absence of WMR-K was performed. Comparing to the untreated dual species biofilm culture, the metabolomic profile of the WMR-K treated culture appears significantly altered. The differentially expressed compounds are mainly related to the primary metabolic pathways, including amino acids, trehalose, pyruvic acid, glycerol and vitamin B6.

Impact of the peptide wmr-k on dual-species biofilm candida albicans/klebsiella pneumoniae and on the untargeted metabolomic profile / Galdiero, E.; Salvatore, M. M.; Maione, A.; Carraturo, F.; Galdiero, S.; Falanga, A.; Andolfi, A.; Salvatore, F.; Guida, M.. - In: PATHOGENS. - ISSN 2076-0817. - 10:2(2021), pp. 1-19. [10.3390/pathogens10020214]

Impact of the peptide wmr-k on dual-species biofilm candida albicans/klebsiella pneumoniae and on the untargeted metabolomic profile

Galdiero E.
Primo
;
Salvatore M. M.
;
Maione A.;Carraturo F.;Galdiero S.;Falanga A.;Andolfi A.;Salvatore F.;Guida M.
Ultimo
2021

Abstract

In recent years, the scientific community has focused on the development of new antibiotics to address the difficulties linked to biofilm-forming microorganisms and drug-resistant infections. In this respect, synthetic antimicrobial peptides (AMPs) are particularly regarded for their therapeutic potential against a broad spectrum of pathogens. In this work, the antimicrobial and antibiofilm activities of the peptide WMR-K towards single and dual species cultures of Candida albicans and Klebsiella pneumoniae were investigated. We found minimum inhibitory concentration (MIC) values for WMR-K of 10 µM for K. pneumoniae and of 200 µM for C. albicans. Furthermore, sub-MIC concentrations of peptide showed an in vitro inhibition of biofilm formation of mono and polymicrobial systems and also a good biofilm eradication even if higher concentrations of it are needed. In order to provide additional evidence for the effect of the examined peptide, a study of changes in extracellular metabolites excreted and/or uptaken from the culture medium (metabolomic footprinting) in the poly-microbial association of C. albicans and K. pneumoniae in presence and absence of WMR-K was performed. Comparing to the untreated dual species biofilm culture, the metabolomic profile of the WMR-K treated culture appears significantly altered. The differentially expressed compounds are mainly related to the primary metabolic pathways, including amino acids, trehalose, pyruvic acid, glycerol and vitamin B6.
2021
Impact of the peptide wmr-k on dual-species biofilm candida albicans/klebsiella pneumoniae and on the untargeted metabolomic profile / Galdiero, E.; Salvatore, M. M.; Maione, A.; Carraturo, F.; Galdiero, S.; Falanga, A.; Andolfi, A.; Salvatore, F.; Guida, M.. - In: PATHOGENS. - ISSN 2076-0817. - 10:2(2021), pp. 1-19. [10.3390/pathogens10020214]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/854341
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