The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high-quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre-analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre-analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.

Next generation sequencing in cytology / Pisapia, Pasquale; Pepe, Francesco; Sgariglia, Roberta; Nacchio, Mariantonia; Russo, Gianluca; Conticelli, Floriana; Girolami, Ilaria; Eccher, Albino; Bellevicine, Claudio; Vigliar, Elena; Malapelle, Umberto; Troncone, Giancarlo. - In: CYTOPATHOLOGY. - ISSN 0956-5507. - (2021). [10.1111/cyt.12974]

Next generation sequencing in cytology

Pisapia, Pasquale;Pepe, Francesco;Sgariglia, Roberta;Nacchio, Mariantonia;Russo, Gianluca;Conticelli, Floriana;Bellevicine, Claudio;Vigliar, Elena;Malapelle, Umberto;Troncone, Giancarlo
2021

Abstract

The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high-quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre-analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre-analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.
2021
Next generation sequencing in cytology / Pisapia, Pasquale; Pepe, Francesco; Sgariglia, Roberta; Nacchio, Mariantonia; Russo, Gianluca; Conticelli, Floriana; Girolami, Ilaria; Eccher, Albino; Bellevicine, Claudio; Vigliar, Elena; Malapelle, Umberto; Troncone, Giancarlo. - In: CYTOPATHOLOGY. - ISSN 0956-5507. - (2021). [10.1111/cyt.12974]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/848978
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