Subsequent inclusion/exclusion criteria to select the best species for an experiment performed on the ISS in a refurbished hardware

The interest in re-using flown hardware for new and different space biology experiments is increasing. To match the constraints of the flown hardware with the requirements of the new biological system, innovative methodological approaches are necessary. MULTITROP was a successful plant biology experiment that was performed on the ISS to investigate multiple-tropism interactions during the early stage of seedling growth. We used the hardware designed and flown for the IFOAM experiment in 2009. The main challenge was to implement seeds of a crop species in a growth chamber conceived for yeast culture and to grow the seedlings in microgravity condition but activating seed germination on ground before the launch. Our approach was to adapt the biological system to the hardware constraints and also to the experiment timing and the environmental factors expected during the prelaunch, launch and flight operations. We looked for an objective and repeatable method to effectively select the best suited species. Innovatively, we applied the method of inclusion/exclusion criteria to adapt a new biological system to a reused hardware. The list and the consecutive order of the specific inclusive/exclusive criteria turned out to be a valid support to guide the science team in objectively choosing the most suitable species for the experiment. Among the 50 initial food species, the carrot seeds resulted as the best in satisfying all technical requirements and post-flight data confirmed the expectations.