Fe-Mimochrome VI*a is a synthetic peroxidase and peroxygenase, featuring two different peptides that are covalently-linked to deuteroheme. To perform a systematic structure/function correlation, we purposely shortened the distance between the distal peptide and the heme, allowing for the separation and characterization of two regioisomers. They differ in both His axial-ligand orientation, as determined by paramagnetic NMR shifts, and activity. These findings highlight that synthetic metalloenzymes may provide an efficient tool for disentangling the role of axial ligand orientation over peroxidase activity.

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts / Maglio, Ornella; Chino, Marco; Vicari, Claudia; Pavone, Vincenzo; Louro, Ricardo O.; Lombardi, Angelina. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - (2021). [10.1039/D0CC06676A]

Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts

Marco Chino
Secondo
Membro del Collaboration Group
;
Vincenzo Pavone
Writing – Review & Editing
;
Angelina Lombardi
Ultimo
Supervision
2021

Abstract

Fe-Mimochrome VI*a is a synthetic peroxidase and peroxygenase, featuring two different peptides that are covalently-linked to deuteroheme. To perform a systematic structure/function correlation, we purposely shortened the distance between the distal peptide and the heme, allowing for the separation and characterization of two regioisomers. They differ in both His axial-ligand orientation, as determined by paramagnetic NMR shifts, and activity. These findings highlight that synthetic metalloenzymes may provide an efficient tool for disentangling the role of axial ligand orientation over peroxidase activity.
2021
Histidine orientation in artificial peroxidase regioisomers as determined by paramagnetic NMR shifts / Maglio, Ornella; Chino, Marco; Vicari, Claudia; Pavone, Vincenzo; Louro, Ricardo O.; Lombardi, Angelina. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - (2021). [10.1039/D0CC06676A]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/828838
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