Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored.

The Union is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm / Papaianni, Marina; Ricciardelli, Annarita; Casillo, Angela; Corsaro, MARIA MICHELA; Borbone, Fabio; DELLA VENTURA, Bartolomeo; Velotta, Raffaele; Fulgione, Andrea; Woo, SHERIDAN LOIS; Tutino, MARIA LUISA; Parrilli, Ermenegilda; Capparelli, Rosanna. - In: MICROORGANISMS. - ISSN 2076-2607. - (2021).

The Union is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm

Papaianni Marina;Ricciardelli annarita;Casillo angela;Corsaro Maria Michela;Borbone fabio;Della Ventura Bartolomeo;Velotta Raffaele;Fulgione Andrea;Woo Sheridan Lois;Tutino Maria Luisa;Parrilli Ermenegilda
;
Capparelli Rosanna
2021

Abstract

Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored.
2021
The Union is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm / Papaianni, Marina; Ricciardelli, Annarita; Casillo, Angela; Corsaro, MARIA MICHELA; Borbone, Fabio; DELLA VENTURA, Bartolomeo; Velotta, Raffaele; Fulgione, Andrea; Woo, SHERIDAN LOIS; Tutino, MARIA LUISA; Parrilli, Ermenegilda; Capparelli, Rosanna. - In: MICROORGANISMS. - ISSN 2076-2607. - (2021).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/828753
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