Background: Allergen-specific immunotherapy (AIT) is a disease-modifying treatment that induces long-term T cell tolerance. Objective: To evaluate the role of circulating CXCR5+ PD-1+. T follicular helper (cTFH) and T. follicular regulatory (TFR) cells following grass pollen subcutaneous (SCIT) and sublingual (SLIT) immunotherapy and the accompanying changes in their chromatin landscape. Methods: Phenotype and function of cTFH cells were initially evaluated in grass pollen-allergics (GPA, n= 28) and non-atopic controls (NAC, n=13) by mathematical algorithms developed to manage high-dimensional data and cell culture, respectively. cTFH and TFR cells were further enumerated in NAC (n=12), GPA (n=14), SCIT (n=10) and SLIT (n=8)-treated groups. Chromatin accessibility in cTFH and TFR cells was assessed by ATAC-seq to investigate epigenetic mechanisms underlying the differences between NAC, GPA, SCIT and SLIT. Results: cTFH cells were shown to be distinct from TH2 and TH2A cell subsets, capable of secreting. IL-4 and IL-21. Both cytokines synergistically promoted B cell class switching to IgE and plasma cell differentiation. Grass pollen allergen induced cTFH cell proliferation in GPA but not in NAC (P<.05). cTFH cells were higher in GPA compared to NAC and were lower in SCIT and SLIT (P<.01). Time91 dependent induction of IL-4, IL-21 and IL-6 were observed in nasal mucosa following intranasal allergen challenge in GPA but not in SCIT and SLIT groups. TFR and IL-10+ 92 cTFH cells were induced 93 in SCIT and SLIT (all, P<.01). ATAC-seq analyses revealed differentially accessible chromatin 94 regions in all groups. Conclusion: For the first time, we showed dysregulation of cTFH cells in GPA compared to NAC, SCIT and SLIT and induction of TFR and IL-10+ 96 cTFH cells following SCIT and SLIT. Changes in the 97 chromatin landscape were observed following AIT in cTFH and TFR cells.
Altered chromatin landscape in circulating T follicular helper and regulatory cells following grass pollen subcutaneous and sublingual immunotherapy / Sharif, Hanisah; Acharya, Swati; Dhondalay, Gopal Krishna R.; Varricchi, Gilda; Krasner-Macleod, Shoshanna; Laisuan, Wannada; Switzer, Amy; Lenormand, Madison; Kashe, Elena; Parkin, Rebecca V.; Zhang, Yi; Koc, Merve; Fedina, Oleksandra; Vilà-Nadal, Gemma; Marone, Gianni; Eifan, Aarif; Scadding, Guy W.; Fear, David J.; Nadeau, Kari C.; Durham, Stephen R.; Shamji, Mohamed H.. - In: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. - ISSN 0091-6749. - 147:2(2021), pp. 663-676. [10.1016/j.jaci.2020.10.035]
Altered chromatin landscape in circulating T follicular helper and regulatory cells following grass pollen subcutaneous and sublingual immunotherapy.
Varricchi, Gilda;Yi, Yi;Marone, Gianni;
2021
Abstract
Background: Allergen-specific immunotherapy (AIT) is a disease-modifying treatment that induces long-term T cell tolerance. Objective: To evaluate the role of circulating CXCR5+ PD-1+. T follicular helper (cTFH) and T. follicular regulatory (TFR) cells following grass pollen subcutaneous (SCIT) and sublingual (SLIT) immunotherapy and the accompanying changes in their chromatin landscape. Methods: Phenotype and function of cTFH cells were initially evaluated in grass pollen-allergics (GPA, n= 28) and non-atopic controls (NAC, n=13) by mathematical algorithms developed to manage high-dimensional data and cell culture, respectively. cTFH and TFR cells were further enumerated in NAC (n=12), GPA (n=14), SCIT (n=10) and SLIT (n=8)-treated groups. Chromatin accessibility in cTFH and TFR cells was assessed by ATAC-seq to investigate epigenetic mechanisms underlying the differences between NAC, GPA, SCIT and SLIT. Results: cTFH cells were shown to be distinct from TH2 and TH2A cell subsets, capable of secreting. IL-4 and IL-21. Both cytokines synergistically promoted B cell class switching to IgE and plasma cell differentiation. Grass pollen allergen induced cTFH cell proliferation in GPA but not in NAC (P<.05). cTFH cells were higher in GPA compared to NAC and were lower in SCIT and SLIT (P<.01). Time91 dependent induction of IL-4, IL-21 and IL-6 were observed in nasal mucosa following intranasal allergen challenge in GPA but not in SCIT and SLIT groups. TFR and IL-10+ 92 cTFH cells were induced 93 in SCIT and SLIT (all, P<.01). ATAC-seq analyses revealed differentially accessible chromatin 94 regions in all groups. Conclusion: For the first time, we showed dysregulation of cTFH cells in GPA compared to NAC, SCIT and SLIT and induction of TFR and IL-10+ 96 cTFH cells following SCIT and SLIT. Changes in the 97 chromatin landscape were observed following AIT in cTFH and TFR cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
