Nucleic acid aptamers are innovative and promising candidates to block the hallmark event in the prion diseases, that is the conversion of prion protein (PrP) into an abnormal form; however, they need chemical modifications for effective therapeutic activity. This communication reports on the development and biophysical characterization of a small library of chemically modified G-quadruplex-forming aptamers targeting the cellular PrP and the evaluation of their anti-prion activity. The results show the possibility of enhancing anti-prion aptamer properties through straightforward modifications.
Improved Anti-Prion Nucleic Acid Aptamers by Incorporation of Chemical Modifications / Amato, Jussara; Mashima, Tsukasa; Kamatari, Yuji O; Kuwata, Kazuo; Novellino, Ettore; Randazzo, Antonio; Giancola, Concetta; Katahira, Masato; Pagano, Bruno. - In: NUCLEIC ACID THERAPEUTICS. - ISSN 2159-3337. - 30:6(2020), pp. 414-421. [10.1089/nat.2020.0899]
Improved Anti-Prion Nucleic Acid Aptamers by Incorporation of Chemical Modifications
Amato, JussaraPrimo
;Novellino, Ettore;Randazzo, Antonio;Giancola, Concetta;Pagano, Bruno
Ultimo
2020
Abstract
Nucleic acid aptamers are innovative and promising candidates to block the hallmark event in the prion diseases, that is the conversion of prion protein (PrP) into an abnormal form; however, they need chemical modifications for effective therapeutic activity. This communication reports on the development and biophysical characterization of a small library of chemically modified G-quadruplex-forming aptamers targeting the cellular PrP and the evaluation of their anti-prion activity. The results show the possibility of enhancing anti-prion aptamer properties through straightforward modifications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
