The study aimed to evaluate if the sperm telomere length can be considered as a new biomarker for sperm quality in bulls. Sperm Telomere Length was evaluated by Monochrome Multiplex Quantitative PCR in group A (n = 8) and group B (n = 8) bulls, classified according to standard semen analysis. Also, this parameter was measured before and after Percoll gradient separation within bulls that produced semen of satisfactory quality. Sperm telomere length, measured as T/S ratio (average ratio of telomere repeats copy number to a single copy gene), was higher in group A than in group B bulls (0.77 ± 0.03 vs 0.43 ± 0.06; P < 0.01). Sperm telomere length was positively correlated with motility, viability and membrane integrity, and it was negatively correlated with sperm anomalies. Furthermore, Percoll gradient selected sperms with higher T/S ratio than unselected sperms (1.19 ± 0.02 vs 0.67 ± 0.03). These results suggest that sperm telomere length can be used as a new marker of bovine semen quality.

Evaluation of bovine sperm telomere length and association with semen quality / Iannuzzi, A.; Della Valle, G.; Russo, M.; Longobardi, V.; Albero, G.; De Canditiis, C.; Kosior, M. A.; Pistucci, R.; Gasparrini, B.. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 158:(2020), pp. 227-232. [10.1016/j.theriogenology.2020.09.019]

Evaluation of bovine sperm telomere length and association with semen quality

Della Valle G.;Russo M.;Longobardi V.;Albero G.;De Canditiis C.;Kosior M. A.;Gasparrini B.
2020

Abstract

The study aimed to evaluate if the sperm telomere length can be considered as a new biomarker for sperm quality in bulls. Sperm Telomere Length was evaluated by Monochrome Multiplex Quantitative PCR in group A (n = 8) and group B (n = 8) bulls, classified according to standard semen analysis. Also, this parameter was measured before and after Percoll gradient separation within bulls that produced semen of satisfactory quality. Sperm telomere length, measured as T/S ratio (average ratio of telomere repeats copy number to a single copy gene), was higher in group A than in group B bulls (0.77 ± 0.03 vs 0.43 ± 0.06; P < 0.01). Sperm telomere length was positively correlated with motility, viability and membrane integrity, and it was negatively correlated with sperm anomalies. Furthermore, Percoll gradient selected sperms with higher T/S ratio than unselected sperms (1.19 ± 0.02 vs 0.67 ± 0.03). These results suggest that sperm telomere length can be used as a new marker of bovine semen quality.
2020
Evaluation of bovine sperm telomere length and association with semen quality / Iannuzzi, A.; Della Valle, G.; Russo, M.; Longobardi, V.; Albero, G.; De Canditiis, C.; Kosior, M. A.; Pistucci, R.; Gasparrini, B.. - In: THERIOGENOLOGY. - ISSN 0093-691X. - 158:(2020), pp. 227-232. [10.1016/j.theriogenology.2020.09.019]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/818923
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