The Nitrogen-Fixing Bacteria—Effective Enhancers of Growth and Chemical Composition of Egyptian Henbane under Varied Mineral N Nutrition

Egyptian henbane (Hyoscyamus muticus L.) plants are rich sources of alkaloids used in pharmaceutical products. Recently, rising eorts have been devoted to reducing mineral fertilizer supply, production cost, and environmental pollution via decreasing the doses of nitrogenous fertilizers and adopting biofertilizer farming systems. Two field experiments were conducted to examine the potential role of N fixing bacteria Azotobacter spp. and Azospirillum spp. on the growth, mineral status, tropane alkaloids, leaf anatomy, and seed yield of Egyptian henbane grown with dierent levels of mineral nitrogen fertilizer, i.e., 25%, 50%, and 100% of the recommended dose, equal to 30, 60, and 120 kg N ha1. N fertilizer improved growth, mineral elements, tropane alkaloids, seed yield, and yield components of Egyptian henbane, which showed a gradually rising trend as the rate of N fertilizer increased. High doses of N fertilizer presumably elicited favorable changes in the anatomical structure of Egyptian henbane leaves. The application of 50% N dose plus N fixing bacteria aected Egyptian henbane trials similarly to 100% of recommended N dose. In conclusion, the N fixing bacteria proved to be a sustainable tool for a two-fold reduction in the recommended dose of mineral N fertilizer and the sustainable management of Egyptian henbane nutrition.