CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing / Ingegnere, T.; Mariotti, F. R.; Pelosi, A.; Quintarelli, C.; De Angelis, B.; Tumino, N.; Besi, F.; Cantoni, C.; Locatelli, F.; Vacca, P.; Moretta, L.. - In: FRONTIERS IN IMMUNOLOGY. - ISSN 1664-3224. - 10:APR(2019), p. 957. [10.3389/fimmu.2019.00957]
Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing
Quintarelli C.;De Angelis B.;Locatelli F.;Vacca P.;
2019
Abstract
CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.| File | Dimensione | Formato | |
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