Diatoms are ubiquitous microalgae that have developed remarkable metabolic plasticity and gene diversification. Here we report the first elucidation of the complete biosynthesis of sterols in the lineage. The study has been carried out on the bloom-forming species Skeletonema marinoi and Cyclotella cryptica that synthesise an ensemble of sterols with chemotypes of animals (cholesterol and desmosterol), plants (dihydrobrassicasterol and 24-methylene cholesterol), algae (fucosterol) and marine invertebrates (clionasterol). In both species, sterols derive from mevalonate through cyclization of squalene to cycloartenol by cycloartenol synthase. The pathway anticipates synthesis of cholesterol by enzymes of the phytosterol route in plants, as recently reported in Solanaceae. Major divergences stem from reduction of Δ24(28) and Δ24(25) double bonds which, in diatoms, are apparently dependent on sterol reductases of fungi, algae and animals. Phylogenetic comparison revealed a good level of similarity between the sterol biosynthetic genes of S. marinoi and C. cryptica with those in the genomes of the other diatoms sequenced so far.

Diatoms synthesize sterols by inclusion of animal and fungal genes in the plant pathway / Gallo, Carmela; Landi, Simone; D’Ippolito, Giuliana; Nuzzo, Genoveffa; Manzo, Emiliano; Sardo &, Angela; Fontana, Angelo. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 10:(2020). [10.1038/s41598-020-60993-5]

Diatoms synthesize sterols by inclusion of animal and fungal genes in the plant pathway

Carmela Gallo
Co-primo
;
Simone Landi
Co-primo
;
Emiliano Manzo;Angelo Fontana
Ultimo
2020

Abstract

Diatoms are ubiquitous microalgae that have developed remarkable metabolic plasticity and gene diversification. Here we report the first elucidation of the complete biosynthesis of sterols in the lineage. The study has been carried out on the bloom-forming species Skeletonema marinoi and Cyclotella cryptica that synthesise an ensemble of sterols with chemotypes of animals (cholesterol and desmosterol), plants (dihydrobrassicasterol and 24-methylene cholesterol), algae (fucosterol) and marine invertebrates (clionasterol). In both species, sterols derive from mevalonate through cyclization of squalene to cycloartenol by cycloartenol synthase. The pathway anticipates synthesis of cholesterol by enzymes of the phytosterol route in plants, as recently reported in Solanaceae. Major divergences stem from reduction of Δ24(28) and Δ24(25) double bonds which, in diatoms, are apparently dependent on sterol reductases of fungi, algae and animals. Phylogenetic comparison revealed a good level of similarity between the sterol biosynthetic genes of S. marinoi and C. cryptica with those in the genomes of the other diatoms sequenced so far.
2020
Diatoms synthesize sterols by inclusion of animal and fungal genes in the plant pathway / Gallo, Carmela; Landi, Simone; D’Ippolito, Giuliana; Nuzzo, Genoveffa; Manzo, Emiliano; Sardo &, Angela; Fontana, Angelo. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 10:(2020). [10.1038/s41598-020-60993-5]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/806387
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