Abstract BACKGROUND: Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates. RESULTS: In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture. CONCLUSIONS: Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.

Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation / Aulitto, Martina; Fusco, Salvatore; Benjamin Nickel, David; Bartolucci, Simonetta; Contursi, Patrizia; Johan Franzén, Carl. - In: BIOTECHNOLOGY FOR BIOFUELS. - ISSN 1754-6834. - (2019). [10.1186/s13068-019-1382-2]

Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation.

Martina Aulitto
Membro del Collaboration Group
;
Salvatore Fusco
Membro del Collaboration Group
;
Simonetta Bartolucci
Membro del Collaboration Group
;
Patrizia Contursi
Membro del Collaboration Group
;
2019

Abstract

Abstract BACKGROUND: Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect microbial growth and metabolism are released from the biomass upon thermochemical pre-treatment. So far, susceptibility of bacterial strains to biomass-derived inhibitors still represents a major barrier to lactic acid production from lignocellulose. Detoxification of the pre-treated lignocellulosic material by water washing is commonly performed to alleviate growth inhibition of the production microorganism and achieve higher production rates. RESULTS: In this study, we assessed the feasibility of replacing the washing step with integrated cellular adaptation during pre-culture of Bacillus coagulans MA-13 prior to simultaneous saccharification and lactic acid fermentation of steam exploded wheat straw. Using a seed culture pre-exposed to 30% hydrolysate led to 50% shorter process time, 50% higher average volumetric and 115% higher average specific productivity than when using cells from a hydrolysate-free seed culture. CONCLUSIONS: Pre-exposure of B. coagulans MA-13 to hydrolysate supports adaptation to the actual production medium. This strategy leads to lower process water requirements and combines cost-effective seed cultivation with physiological pre-adaptation of the production strain, resulting in reduced lactic acid production costs.
2019
Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation / Aulitto, Martina; Fusco, Salvatore; Benjamin Nickel, David; Bartolucci, Simonetta; Contursi, Patrizia; Johan Franzén, Carl. - In: BIOTECHNOLOGY FOR BIOFUELS. - ISSN 1754-6834. - (2019). [10.1186/s13068-019-1382-2]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/788355
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