The Heat Shock Proteins (HSPs) are a superfamily of molecular chaperones that maintain cellular homeostasis under stress. HSP70 represents the major stress-inducible family member, often activated in response to changes in thermal ranges of organisms, and therefore playing an important role enhancing thermal tolerance limits in ectothermic animals. The present study aimed to investigate the presence and the localization of HSP70 through the development of Podarcis siculus, an oviparous lizard inhabiting temperate Mediterranean regions, showing a limited potential to tolerate thermal changes during embryogenesis. Immunohistochemical analysis demonstrated that HSP70 protein is constitutively present in early embryonic stages, abundantly distributed in eye, in encephalic domains (predominantly in ventricular areas and in grey matter), in grey matter of spinal cord, in lung, gut mucosa, hepatic cords and kidney tubules. Interestingly, a severe drop in incubation temperature (5 8C for 3 days) does not induce enhancements in HSP70 levels nor changes in tissues localization. These results suggest that the HSP70 found in P. siculus embryos represents a non-inducible, constitutive molecular chaperone that should be better called Heat Shock Cognate 70 (HSC70); the presence of stress- induced members of the HSP family in P. siculus has yet to be proven.

HSP70 localization in Podarcis siculus embryos under natural thermal regime and following a non-lethal cold shock / Scudiero, Rosaria; Verderame, Mariailaria; Motta, CHIARA MARIA; Migliaccio, Vincenzo; Simoniello, Palma. - In: COMPTES RENDUS BIOLOGIES. - ISSN 1631-0691. - 342:9-10(2019), pp. 299-308. [10.1016/j.crvi.2019.10.001]

HSP70 localization in Podarcis siculus embryos under natural thermal regime and following a non-lethal cold shock

Rosaria Scudiero
Primo
;
Chiara Maria Motta;Vincenzo Migliaccio
Penultimo
;
Palma Simoniello
Ultimo
2019

Abstract

The Heat Shock Proteins (HSPs) are a superfamily of molecular chaperones that maintain cellular homeostasis under stress. HSP70 represents the major stress-inducible family member, often activated in response to changes in thermal ranges of organisms, and therefore playing an important role enhancing thermal tolerance limits in ectothermic animals. The present study aimed to investigate the presence and the localization of HSP70 through the development of Podarcis siculus, an oviparous lizard inhabiting temperate Mediterranean regions, showing a limited potential to tolerate thermal changes during embryogenesis. Immunohistochemical analysis demonstrated that HSP70 protein is constitutively present in early embryonic stages, abundantly distributed in eye, in encephalic domains (predominantly in ventricular areas and in grey matter), in grey matter of spinal cord, in lung, gut mucosa, hepatic cords and kidney tubules. Interestingly, a severe drop in incubation temperature (5 8C for 3 days) does not induce enhancements in HSP70 levels nor changes in tissues localization. These results suggest that the HSP70 found in P. siculus embryos represents a non-inducible, constitutive molecular chaperone that should be better called Heat Shock Cognate 70 (HSC70); the presence of stress- induced members of the HSP family in P. siculus has yet to be proven.
2019
HSP70 localization in Podarcis siculus embryos under natural thermal regime and following a non-lethal cold shock / Scudiero, Rosaria; Verderame, Mariailaria; Motta, CHIARA MARIA; Migliaccio, Vincenzo; Simoniello, Palma. - In: COMPTES RENDUS BIOLOGIES. - ISSN 1631-0691. - 342:9-10(2019), pp. 299-308. [10.1016/j.crvi.2019.10.001]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/779791
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