Middle age is an early stage of the aging process, during which the consumption of diets rich in saturated fats and/or simple sugars might influence brain function, but only few data are available on this issue.We therefore investigated the impact of a diet rich in saturated fat and fructose (HFF) on mitochondrial physiology in hippocampus and frontal cortex of middle-aged rats (1 year old), by including a group of adult rats (90 days) as a Bnegative control,^ lacking the putative effect of aging.Middle-aged rats were fed HFF or control diet for 4 weeks. Mitochondrial function was analyzed by high-resolution respirometry and by assessing the amount of respiratory complexes. Markers of oxidative balance, as well as the protein content of uncoupling protein 2 (UCP2), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and peroxisome proliferator-activated receptor alpha (PPARα), were also assessed. A decrease in the activity of complex I was detected in both brain areas ofmiddle-aged rats. In hippocampus,mitochondrial respiratory capacity and complex IV content decreasedwith age and increased with HFF diet. Higher protein oxidative damage, decreased antioxidant defenses, and increased UCP2 and PGC-1α content were found in hippocampus of middle-aged rats. HFF feeding induced a significant reduction in the amount of UCP2, PGC-1α, and PPARα, together with higher protein oxidative damage, in both brain areas. Overall, our results point to middle age as a condition of early brain aging for mitochondrial function, with hippocampus being an area more susceptible to metabolic impairment than frontal cortex.

Effect of Initial Aging and High-Fat/High-Fructose Diet on Mitochondrial Bioenergetics and Oxidative Status in Rat Brain / Crescenzo, Raffaella; Spagnuolo, MARIA STEFANIA; Cancelliere, Rosa; Iannotta, Lucia; Mazzoli, Arianna; Gatto, Cristina; Iossa, Susanna; Cigliano, Luisa. - In: MOLECULAR NEUROBIOLOGY. - ISSN 0893-7648. - 56:(2019), pp. 7651-7663. [10.1007/s12035-019-1617-z]

Effect of Initial Aging and High-Fat/High-Fructose Diet on Mitochondrial Bioenergetics and Oxidative Status in Rat Brain

Raffaella Crescenzo
Primo
;
Maria Stefania Spagnuolo;Rosa Cancelliere;Lucia Iannotta;Arianna Mazzoli;GATTO, CRISTINA;Susanna Iossa
Penultimo
;
Luisa Cigliano
Ultimo
2019

Abstract

Middle age is an early stage of the aging process, during which the consumption of diets rich in saturated fats and/or simple sugars might influence brain function, but only few data are available on this issue.We therefore investigated the impact of a diet rich in saturated fat and fructose (HFF) on mitochondrial physiology in hippocampus and frontal cortex of middle-aged rats (1 year old), by including a group of adult rats (90 days) as a Bnegative control,^ lacking the putative effect of aging.Middle-aged rats were fed HFF or control diet for 4 weeks. Mitochondrial function was analyzed by high-resolution respirometry and by assessing the amount of respiratory complexes. Markers of oxidative balance, as well as the protein content of uncoupling protein 2 (UCP2), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and peroxisome proliferator-activated receptor alpha (PPARα), were also assessed. A decrease in the activity of complex I was detected in both brain areas ofmiddle-aged rats. In hippocampus,mitochondrial respiratory capacity and complex IV content decreasedwith age and increased with HFF diet. Higher protein oxidative damage, decreased antioxidant defenses, and increased UCP2 and PGC-1α content were found in hippocampus of middle-aged rats. HFF feeding induced a significant reduction in the amount of UCP2, PGC-1α, and PPARα, together with higher protein oxidative damage, in both brain areas. Overall, our results point to middle age as a condition of early brain aging for mitochondrial function, with hippocampus being an area more susceptible to metabolic impairment than frontal cortex.
2019
Effect of Initial Aging and High-Fat/High-Fructose Diet on Mitochondrial Bioenergetics and Oxidative Status in Rat Brain / Crescenzo, Raffaella; Spagnuolo, MARIA STEFANIA; Cancelliere, Rosa; Iannotta, Lucia; Mazzoli, Arianna; Gatto, Cristina; Iossa, Susanna; Cigliano, Luisa. - In: MOLECULAR NEUROBIOLOGY. - ISSN 0893-7648. - 56:(2019), pp. 7651-7663. [10.1007/s12035-019-1617-z]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/766011
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