Waste biomass from agro-food industry can be exploited to produce several products according to the sugar-based biorefinery concept. The success of the biomass exploitation stems from the equilibrium among severalissues regarding the process costs, the environmental impact, and the energy efficiency. The present work isfocused on the use of apple pomace as possible renewable feedstock for the production of biofuels and of bio-commodities. The aim of the present work was the optimization of the enzymatic pretreatment and hydrolysis ofapple pomace in a bubble column bioreactor by using a recombinant laccase as biocatalyst for the delignificationstep and commercial cellulases for the hydrolysis step. Both the enzymatic processes were investigated to selectthe optimal operating conditions for the maximization of sugar yields. Optimal conditions for the sequentialenzymatic delignification and hydrolysis process in the bubble column were: 15%w/v biomass concentration;60 nL/h airflow rate, 30 U/gbiomasslaccase activity; 20 FPU/gcellulosecellulase activity. Moreover, the selectedoperating conditions were applied to a long term test of the sequential pretreatment/hydrolysis in the bubblecolumn that resulted in a maximum sugar yield of 0.34 gsugars/graw biomassand 0.61 gsugars/gsugars in the pretreatedbiomassfrom the raw apple pomace. Result discussion highlighted advantages and drawback of pneumatic mixingand sequential pretreatment/hydrolysis strategy applied to enzymatic delignification and cellulose hydrolysis.
Integrated enzymatic pretreatment and hydrolysis of apple pomace in a bubble column bioreactor / Niglio, S.; Procentese, A.; Russo, M. E.; Piscitelli, A.; Marzocchella, A.. - In: BIOCHEMICAL ENGINEERING JOURNAL. - ISSN 1369-703X. - 150:(2019), p. 107306. [10.1016/j.bej.2019.107306]
Integrated enzymatic pretreatment and hydrolysis of apple pomace in a bubble column bioreactor
Niglio S.Investigation
;Piscitelli A.Membro del Collaboration Group
;Marzocchella A.Membro del Collaboration Group
2019
Abstract
Waste biomass from agro-food industry can be exploited to produce several products according to the sugar-based biorefinery concept. The success of the biomass exploitation stems from the equilibrium among severalissues regarding the process costs, the environmental impact, and the energy efficiency. The present work isfocused on the use of apple pomace as possible renewable feedstock for the production of biofuels and of bio-commodities. The aim of the present work was the optimization of the enzymatic pretreatment and hydrolysis ofapple pomace in a bubble column bioreactor by using a recombinant laccase as biocatalyst for the delignificationstep and commercial cellulases for the hydrolysis step. Both the enzymatic processes were investigated to selectthe optimal operating conditions for the maximization of sugar yields. Optimal conditions for the sequentialenzymatic delignification and hydrolysis process in the bubble column were: 15%w/v biomass concentration;60 nL/h airflow rate, 30 U/gbiomasslaccase activity; 20 FPU/gcellulosecellulase activity. Moreover, the selectedoperating conditions were applied to a long term test of the sequential pretreatment/hydrolysis in the bubblecolumn that resulted in a maximum sugar yield of 0.34 gsugars/graw biomassand 0.61 gsugars/gsugars in the pretreatedbiomassfrom the raw apple pomace. Result discussion highlighted advantages and drawback of pneumatic mixingand sequential pretreatment/hydrolysis strategy applied to enzymatic delignification and cellulose hydrolysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
