The development of in vitro embryo production (IVEP) techniques in Felis catus is a fitting model with potential application to the conservation of endangered felid species. To improve the quality of IVEP techniques an appropriate balance of pro- and antioxidants should be provided. Under in vitro conditions, high levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) mRNA provide a defence mechanism against oxidative stress for embryos. In order to improve the development of cat oocytes, the effects of SOD and CAT supplemented to in vitro maturation (IVM) medium and of GPx supplemented to in vitro fertilization (IVF) medium on development and embryo production in vitro were evaluated. Data showed an increase of 70 and 77 % of cleaved embryo and blastocyst formation, respectively, in the experiment with SOD and CAT addition to IVM medium; in the experiment with GPx addition to IVF medium the number of cleaved embryos doubled and the number of embryos increased by 96 %. Therefore, our results were positive and encourage us to continue studies on cat oocytes evaluating the effects of various dosages and combination of antioxidants.

Antioxidant supplementation to medium for in vitro embryo production in Felis catus / Cocchia, N.; Tafuri, S.; Del Prete, C.; Palumbo, V.; Esposito, L.; Avallone, L.; Ciani, F.. - In: POLISH JOURNAL OF VETERINARY SCIENCES. - ISSN 1505-1773. - 22(3):(2019), pp. 573-579. [10.24425/pjvs.2019.129966]

Antioxidant supplementation to medium for in vitro embryo production in Felis catus

N. Cocchia;S. Tafuri;C. Del Prete;V. Palumbo;L. Esposito;L. Avallone;F. Ciani
2019

Abstract

The development of in vitro embryo production (IVEP) techniques in Felis catus is a fitting model with potential application to the conservation of endangered felid species. To improve the quality of IVEP techniques an appropriate balance of pro- and antioxidants should be provided. Under in vitro conditions, high levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) mRNA provide a defence mechanism against oxidative stress for embryos. In order to improve the development of cat oocytes, the effects of SOD and CAT supplemented to in vitro maturation (IVM) medium and of GPx supplemented to in vitro fertilization (IVF) medium on development and embryo production in vitro were evaluated. Data showed an increase of 70 and 77 % of cleaved embryo and blastocyst formation, respectively, in the experiment with SOD and CAT addition to IVM medium; in the experiment with GPx addition to IVF medium the number of cleaved embryos doubled and the number of embryos increased by 96 %. Therefore, our results were positive and encourage us to continue studies on cat oocytes evaluating the effects of various dosages and combination of antioxidants.
2019
Antioxidant supplementation to medium for in vitro embryo production in Felis catus / Cocchia, N.; Tafuri, S.; Del Prete, C.; Palumbo, V.; Esposito, L.; Avallone, L.; Ciani, F.. - In: POLISH JOURNAL OF VETERINARY SCIENCES. - ISSN 1505-1773. - 22(3):(2019), pp. 573-579. [10.24425/pjvs.2019.129966]
File in questo prodotto:
File Dimensione Formato  
Cocchia et al. Pol J vet sci 2019.pdf

accesso aperto

Licenza: Accesso privato/ristretto
Dimensione 6.29 MB
Formato Adobe PDF
6.29 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/761462
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact