Carbohydrate-lectin interactions intervene in and mediate most biological processes, including a crucial modulation of immune responses to pathogens. Despite growing interest in investigating the association between host receptor lectins and exogenous glycan ligands, the molecular mechanisms underlying bacterial recognition by human lectins are still not fully understood. Herein, a novel molecular interaction between the human macrophage galactose-type lectin (MGL) and the lipooligosaccharide (LOS) of Escherichia coli strain R1 is described. Saturation transfer difference NMR spectroscopy analysis, supported by computational studies, demonstrated that MGL bound to the purified deacylated LOSR1 mainly through recognition of its outer core and established crucial interactions with the terminal Galα(1,2)Gal epitope. These results assess the ability of MGL to recognise glycan moieties exposed on Gram-negative bacterial surfaces.

Human Macrophage Galactose-Type Lectin (MGL) Recognizes the Outer Core of Escherichia coli Lipooligosaccharide / Maalej, Meriem; Forgione, ROSA ESTER; Marchetti, R.; Bulteau, F.; Thepaut, M.; Lanzetta, R.; Laguri, C.; Simorre, J. -P.; Fieschi, F.; Molinaro, A.; Silipo, A.. - In: CHEMBIOCHEM. - ISSN 1439-4227. - 15:(2019), pp. 1778-1782. [10.1002/cbic.201900087]

Human Macrophage Galactose-Type Lectin (MGL) Recognizes the Outer Core of Escherichia coli Lipooligosaccharide

MAALEJ, MERIEM;FORGIONE, ROSA ESTER;Marchetti R.;Lanzetta R.;Molinaro A.;Silipo A.
2019

Abstract

Carbohydrate-lectin interactions intervene in and mediate most biological processes, including a crucial modulation of immune responses to pathogens. Despite growing interest in investigating the association between host receptor lectins and exogenous glycan ligands, the molecular mechanisms underlying bacterial recognition by human lectins are still not fully understood. Herein, a novel molecular interaction between the human macrophage galactose-type lectin (MGL) and the lipooligosaccharide (LOS) of Escherichia coli strain R1 is described. Saturation transfer difference NMR spectroscopy analysis, supported by computational studies, demonstrated that MGL bound to the purified deacylated LOSR1 mainly through recognition of its outer core and established crucial interactions with the terminal Galα(1,2)Gal epitope. These results assess the ability of MGL to recognise glycan moieties exposed on Gram-negative bacterial surfaces.
2019
Human Macrophage Galactose-Type Lectin (MGL) Recognizes the Outer Core of Escherichia coli Lipooligosaccharide / Maalej, Meriem; Forgione, ROSA ESTER; Marchetti, R.; Bulteau, F.; Thepaut, M.; Lanzetta, R.; Laguri, C.; Simorre, J. -P.; Fieschi, F.; Molinaro, A.; Silipo, A.. - In: CHEMBIOCHEM. - ISSN 1439-4227. - 15:(2019), pp. 1778-1782. [10.1002/cbic.201900087]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/756637
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