Our previous data demonstrated that nucleolar stress induced by anticancer drugs in colon cancer cells devoid of p53 leads to the activation of ribosomal protein L3 as inhibitor of cell cycle. In particular, nucleolar stress induces L3 expression and promote its nucleolar exit. To understand the molecular mechanism underlying this effect, HCT 116p53-/- cells were treated with Actinomycin D to induce nucleolar stress and with cycloheximide to block NMD pathway. Results showed that the delocalization of L3 during nucleolar stress was associated with an increase alternative L3 pre-mRNA, substrate of the NMD. Next, we quantified known cell cycle-associated E2F target genes as CycD1, CycE1 and CDK1 by quantitative RT-PCR in HCT 116p53-/- and L3ΔHCT 116p53-/- cells, in which L3 is stably silenced. We detected a significant up-regulation of E2F target genes associated with L3 knockdown. Results from reporter gene assays in presence and in absence of L3 upon drug- induced nucleolar stress demonstrated that L3 acts as positive regulator of E2F transcriptional activity. In conclusion, L3 regulates cell proliferation independently of p53 by targeting E2F and inhibiting its function.

Ribosome-free L3 regulates cell proliferation independently of p53 by targeting E2F and inhibiting its function / Pecoraro, Annalisa; Olindo Giovanni Esposito, ; Ilaria Di Stefano, ; Rossella Di Domenico, ; Russo, Giulia; Russo, Annapina. - (2018), pp. 94-95. (Intervento presentato al convegno XV FISV CONGRESS tenutosi a roma nel 18-21 settembre).

Ribosome-free L3 regulates cell proliferation independently of p53 by targeting E2F and inhibiting its function

PECORARO, ANNALISA;Giulia Russo;Annapina Russo
2018

Abstract

Our previous data demonstrated that nucleolar stress induced by anticancer drugs in colon cancer cells devoid of p53 leads to the activation of ribosomal protein L3 as inhibitor of cell cycle. In particular, nucleolar stress induces L3 expression and promote its nucleolar exit. To understand the molecular mechanism underlying this effect, HCT 116p53-/- cells were treated with Actinomycin D to induce nucleolar stress and with cycloheximide to block NMD pathway. Results showed that the delocalization of L3 during nucleolar stress was associated with an increase alternative L3 pre-mRNA, substrate of the NMD. Next, we quantified known cell cycle-associated E2F target genes as CycD1, CycE1 and CDK1 by quantitative RT-PCR in HCT 116p53-/- and L3ΔHCT 116p53-/- cells, in which L3 is stably silenced. We detected a significant up-regulation of E2F target genes associated with L3 knockdown. Results from reporter gene assays in presence and in absence of L3 upon drug- induced nucleolar stress demonstrated that L3 acts as positive regulator of E2F transcriptional activity. In conclusion, L3 regulates cell proliferation independently of p53 by targeting E2F and inhibiting its function.
2018
Ribosome-free L3 regulates cell proliferation independently of p53 by targeting E2F and inhibiting its function / Pecoraro, Annalisa; Olindo Giovanni Esposito, ; Ilaria Di Stefano, ; Rossella Di Domenico, ; Russo, Giulia; Russo, Annapina. - (2018), pp. 94-95. (Intervento presentato al convegno XV FISV CONGRESS tenutosi a roma nel 18-21 settembre).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/747225
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