Brahma remodeler plays an important role in maintaining chromatin structure genomewide. We are focused on its role in activated transcription. For this aim we have generated the construct carrying reporter gene under control of lexA-binding sites. The construct was integrated into different loci of Drosophila genome in proximity to different endogenous enhancers. The reporter gene expression was under control of these enhancers and lexA fusions with different Brahma subunits. Only the cells carrying both active enhancer and lexA fusions show reporter expression. Thus, tethering of Brahma subunit at promoter could mediate enhancer-dependent transcriptional activation. Using tissue-specific knockdowns, we are studying re contribution of different transcription factors into activity of the reporter. The system will allow us to study the role of Brahma in transcription activation and its cooperation with transcription machinery in this process.
Studying brahma remodeler functions in long-range interactions / Giordano, E.; Putlyaev, E.; Shaposhnikov, A.; Lebedeva, L.; Schedl, P.; Shidlovskii, Y.. - EMBO Conference: Nuclear structure and dynamics:(2017), pp. 8-8. (Intervento presentato al convegno Nuclear structure and dynamics 2017 tenutosi a L'Isle sur la Sorgue, France nel 04 – 08 Ottobre 2017).
Studying brahma remodeler functions in long-range interactions.
E. Giordano
;
2017
Abstract
Brahma remodeler plays an important role in maintaining chromatin structure genomewide. We are focused on its role in activated transcription. For this aim we have generated the construct carrying reporter gene under control of lexA-binding sites. The construct was integrated into different loci of Drosophila genome in proximity to different endogenous enhancers. The reporter gene expression was under control of these enhancers and lexA fusions with different Brahma subunits. Only the cells carrying both active enhancer and lexA fusions show reporter expression. Thus, tethering of Brahma subunit at promoter could mediate enhancer-dependent transcriptional activation. Using tissue-specific knockdowns, we are studying re contribution of different transcription factors into activity of the reporter. The system will allow us to study the role of Brahma in transcription activation and its cooperation with transcription machinery in this process.File | Dimensione | Formato | |
---|---|---|---|
EMBO 2017.pdf
accesso aperto
Tipologia:
Abstract
Licenza:
Accesso privato/ristretto
Dimensione
323.5 kB
Formato
Adobe PDF
|
323.5 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.