BACKGROUND: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. METHODS: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. RESULTS: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. CONCLUSIONS: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. GENERAL SIGNIFICANCE: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.

TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways / Venuto, Santina; Castellana, Stefano; Monti, Maria; Appolloni, Irene; Fusilli, Caterina; Fusco, Carmela; Pucci, Pietro; Malatesta, Paolo; Mazza, Tommaso; Merla, Giuseppe; Micale, Lucia. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 1863:2(2019), pp. 491-501. [10.1016/j.bbagen.2018.12.001]

TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways

Monti, Maria;Pucci, Pietro;Merla, Giuseppe;
2019

Abstract

BACKGROUND: We recently reported TRIM8, encoding an E3 ubiquitin ligase, as a gene aberrantly expressed in glioblastoma whose expression suppresses cell growth and induces a significant reduction of clonogenic potential in glioblastoma cell lines. METHODS: we provided novel insights on TRIM8 functions by profiling the transcriptome of TRIM8-expressing primary mouse embryonal neural stem cells by RNA-sequencing and bioinformatic analysis. Functional analysis including luciferase assay, western blot, PCR arrays, Real time quantitative PCR were performed to validate the transcriptomic data. RESULTS: Our study identified enriched pathways related to the neurotransmission and to the central nervous system (CNS) functions, including axonal guidance, GABA receptor, Ephrin B, synaptic long-term potentiation/depression, and glutamate receptor signalling pathways. Finally, we provided additional evidence about the existence of a functional interactive crosstalk between TRIM8 and STAT3. CONCLUSIONS: Our results substantiate the role of TRIM8 in the brain functions through the dysregulation of genes involved in different CNS-related pathways, including JAK-STAT. GENERAL SIGNIFICANCE: This study provides novel insights on the physiological TRIM8 function by profiling for the first time the primary Neural Stem Cell over-expressing TRIM8 by using RNA-Sequencing methodology.
2019
TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways / Venuto, Santina; Castellana, Stefano; Monti, Maria; Appolloni, Irene; Fusilli, Caterina; Fusco, Carmela; Pucci, Pietro; Malatesta, Paolo; Mazza, Tommaso; Merla, Giuseppe; Micale, Lucia. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 1863:2(2019), pp. 491-501. [10.1016/j.bbagen.2018.12.001]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/728213
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