Any reference laboratory testing non-small cell lung cancer samples for predictive biomarkers needs to develop and validate a wide range of different molecular techniques, each with a specific time requirement and application. Updated international guidelines suggest that next generation sequencing (NGS) to be the initial procedure. However, in a non-negligible subset of cases, library generation may fail or amplicon coverage may be insufficient. In these NGS 'invalid' cases, the Idylla system may represent a viable option for rapid epidermal growth factor receptor (EGFR) genotyping.

Idylla assay and next generation sequencing: an integrated EGFR mutational testing algorithm / De Luca, Caterina; Rappa, Alessandra G; Gragnano, Gianluca; Malapelle, Umberto; Troncone, Giancarlo; Barberis, Massimo. - In: JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0021-9746. - 71:8(2018), pp. 745-750-750. [10.1136/jclinpath-2018-205197]

Idylla assay and next generation sequencing: an integrated EGFR mutational testing algorithm

De Luca, Caterina;Gragnano, Gianluca;Malapelle, Umberto;Troncone, Giancarlo;
2018

Abstract

Any reference laboratory testing non-small cell lung cancer samples for predictive biomarkers needs to develop and validate a wide range of different molecular techniques, each with a specific time requirement and application. Updated international guidelines suggest that next generation sequencing (NGS) to be the initial procedure. However, in a non-negligible subset of cases, library generation may fail or amplicon coverage may be insufficient. In these NGS 'invalid' cases, the Idylla system may represent a viable option for rapid epidermal growth factor receptor (EGFR) genotyping.
2018
Idylla assay and next generation sequencing: an integrated EGFR mutational testing algorithm / De Luca, Caterina; Rappa, Alessandra G; Gragnano, Gianluca; Malapelle, Umberto; Troncone, Giancarlo; Barberis, Massimo. - In: JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0021-9746. - 71:8(2018), pp. 745-750-750. [10.1136/jclinpath-2018-205197]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/727300
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