Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded resolution due to shared marker expression and atypical heterogeneity in vascular tissues. We applied the high-dimensionality of mass cytometry to the study of myeloid cell subsets in atherosclerosis. Apolipoprotein E-deficient (ApoE-/-) mice were fed a chow or a high fat (western) diet for 12 weeks. Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45þ cells from the aortas of ApoE-/- mice identified 13 broad populations of leucocytes. Monocyte, macrophage, type 1 and type 2 conventional dendritic cell (cDC1 and cDC2), plasmacytoid dendritic cell (pDC), neutrophil, eosinophil, B cell, CD4þ and CD8þ T cell, cd T cell, natural killer (NK) cell, and innate lymphoid cell (ILC) populations accounted for approximately 95% of the live CD45þ aortic cells. Automated clustering algorithms applied to the Lin-CD11blo-hi cells revealed 20 clusters of myeloid cells. Comparison between chow and high fat fed animals revealed increases in monocytes (both Ly6Cþ and Ly6C-), pDC, and a CD11cþ macrophage subset with high fat feeding. Concomitantly, the proportions of CD206þ CD169þ subsets of macrophages were significantly reduced as were cDC2. A CyTOF-based comprehensive mapping of the immune cell subsets within atherosclerotic aortas from ApoE-/- mice offers tools for myeloid cell discrimination within the vascular compartment and it reveals that high fat feeding skews the myeloid cell repertoire toward inflammatory monocyte-macrophage populations rather than resident macrophage phenotypes and cDC2 during atherogenesis.

Immune cell census in murine atherosclerosis: Cytometry by time of flight illuminates vascular myeloid cell diversity / Cole, Jennifer E.; Park, Inhye; Ahern, David J.; Kassiteridi, Christina; Abeam, Dina Danso; Goddard, Michael E.; Green, Patricia; Maffia, Pasquale; Monaco, Claudia. - In: CARDIOVASCULAR RESEARCH. - ISSN 0008-6363. - 114:10(2018), pp. 1360-1371. [10.1093/cvr/cvy109]

Immune cell census in murine atherosclerosis: Cytometry by time of flight illuminates vascular myeloid cell diversity

Maffia, Pasquale
Penultimo
Membro del Collaboration Group
;
2018

Abstract

Atherosclerosis is characterized by the abundant infiltration of myeloid cells starting at early stages of disease. Myeloid cells are key players in vascular immunity during atherogenesis. However, the subsets of vascular myeloid cells have eluded resolution due to shared marker expression and atypical heterogeneity in vascular tissues. We applied the high-dimensionality of mass cytometry to the study of myeloid cell subsets in atherosclerosis. Apolipoprotein E-deficient (ApoE-/-) mice were fed a chow or a high fat (western) diet for 12 weeks. Single-cell aortic preparations were probed with a panel of 35 metal-conjugated antibodies using cytometry by time of flight (CyTOF). Clustering of marker expression on live CD45þ cells from the aortas of ApoE-/- mice identified 13 broad populations of leucocytes. Monocyte, macrophage, type 1 and type 2 conventional dendritic cell (cDC1 and cDC2), plasmacytoid dendritic cell (pDC), neutrophil, eosinophil, B cell, CD4þ and CD8þ T cell, cd T cell, natural killer (NK) cell, and innate lymphoid cell (ILC) populations accounted for approximately 95% of the live CD45þ aortic cells. Automated clustering algorithms applied to the Lin-CD11blo-hi cells revealed 20 clusters of myeloid cells. Comparison between chow and high fat fed animals revealed increases in monocytes (both Ly6Cþ and Ly6C-), pDC, and a CD11cþ macrophage subset with high fat feeding. Concomitantly, the proportions of CD206þ CD169þ subsets of macrophages were significantly reduced as were cDC2. A CyTOF-based comprehensive mapping of the immune cell subsets within atherosclerotic aortas from ApoE-/- mice offers tools for myeloid cell discrimination within the vascular compartment and it reveals that high fat feeding skews the myeloid cell repertoire toward inflammatory monocyte-macrophage populations rather than resident macrophage phenotypes and cDC2 during atherogenesis.
2018
Immune cell census in murine atherosclerosis: Cytometry by time of flight illuminates vascular myeloid cell diversity / Cole, Jennifer E.; Park, Inhye; Ahern, David J.; Kassiteridi, Christina; Abeam, Dina Danso; Goddard, Michael E.; Green, Patricia; Maffia, Pasquale; Monaco, Claudia. - In: CARDIOVASCULAR RESEARCH. - ISSN 0008-6363. - 114:10(2018), pp. 1360-1371. [10.1093/cvr/cvy109]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/726541
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