Salmonella Typhimurium is one of the main causes of outbreaks and sporadic cases of human gastroenteritis. At present, the rapid detection of this pathogen is a major goal of biosensing technology applied to food safety. In fact, ISO standardized culture method takes up to ten days to provide a reliable response. In this paper, we describe a relatively simple protocol for detecting Salmonella Typhimurium in chicken meat based on a Quartz-Crystal Microbalance (QCM), which leads to a limit of detection (LOD) less than of 10° CFU/mL and requires a pre-enrichment step lasting only 2 h at 37 °C. The reliability of the proposed immunosensor has been demonstrated through the validation of the experimental results with ISO standardized culture method. The cost-effectiveness of the procedure and the rapidity of the QCM-based biosensor in providing the qualitative response make the analytical method described here suitable for applications in food inspection laboratory and throughout the chain production of food industry.

QCM-based immunosensor for rapid detection of Salmonella Typhimurium in food / Fulgione, Andrea; Cimafonte, Martina; DELLA VENTURA, Bartolomeo; Iannaccone, Marco; Ambrosino, Concetta; Capuano, Federico; Proroga, Yolande Therese Rose; Velotta, Raffaele; Capparelli, Rosanna. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018), p. 16137. [10.1038/s41598-018-34285-y]

QCM-based immunosensor for rapid detection of Salmonella Typhimurium in food

Andrea Fulgione;Martina Cimafonte;Bartolomeo Della Ventura;Marco Iannaccone;Federico Capuano;Yolande Thérèse Rose Proroga;Raffaele Velotta;Rosanna Capparelli
2018

Abstract

Salmonella Typhimurium is one of the main causes of outbreaks and sporadic cases of human gastroenteritis. At present, the rapid detection of this pathogen is a major goal of biosensing technology applied to food safety. In fact, ISO standardized culture method takes up to ten days to provide a reliable response. In this paper, we describe a relatively simple protocol for detecting Salmonella Typhimurium in chicken meat based on a Quartz-Crystal Microbalance (QCM), which leads to a limit of detection (LOD) less than of 10° CFU/mL and requires a pre-enrichment step lasting only 2 h at 37 °C. The reliability of the proposed immunosensor has been demonstrated through the validation of the experimental results with ISO standardized culture method. The cost-effectiveness of the procedure and the rapidity of the QCM-based biosensor in providing the qualitative response make the analytical method described here suitable for applications in food inspection laboratory and throughout the chain production of food industry.
2018
QCM-based immunosensor for rapid detection of Salmonella Typhimurium in food / Fulgione, Andrea; Cimafonte, Martina; DELLA VENTURA, Bartolomeo; Iannaccone, Marco; Ambrosino, Concetta; Capuano, Federico; Proroga, Yolande Therese Rose; Velotta, Raffaele; Capparelli, Rosanna. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018), p. 16137. [10.1038/s41598-018-34285-y]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/724431
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