Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for designing and engineering artificial enzymes with defined catalytic properties. Here, we report the conjugation of an artificial heme enzyme (MIMO) with lipoic acid (LA) as a building block for the development of gold-based biomaterials. We show that the artificial MIMO@LA can be successfully conjugated to gold nanoparticles or immobilized onto gold electrode surfaces, displaying quasi-reversible redox properties and peroxidase activity. The results of this work open interesting perspectives toward the development of new totally-synthetic catalytic biomaterials for application in biotechnology and biomedicine, expanding the range of the biomolecular component aside from traditional native enzymes.

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials / Zambrano, Gerardo; Ruggiero, Emmanuel; Malafronte, Anna; Chino, Marco; Maglio, Ornella; Pavone, Vincenzo; Nastri, Flavia; Lombardi, Angela. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 19:10(2018), p. 2896. [10.3390/ijms19102896]

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

Gerardo Zambrano;Anna Malafronte;Marco Chino;Ornella Maglio;Vincenzo Pavone;Flavia Nastri
;
Angela Lombardi
2018

Abstract

Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for designing and engineering artificial enzymes with defined catalytic properties. Here, we report the conjugation of an artificial heme enzyme (MIMO) with lipoic acid (LA) as a building block for the development of gold-based biomaterials. We show that the artificial MIMO@LA can be successfully conjugated to gold nanoparticles or immobilized onto gold electrode surfaces, displaying quasi-reversible redox properties and peroxidase activity. The results of this work open interesting perspectives toward the development of new totally-synthetic catalytic biomaterials for application in biotechnology and biomedicine, expanding the range of the biomolecular component aside from traditional native enzymes.
2018
Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials / Zambrano, Gerardo; Ruggiero, Emmanuel; Malafronte, Anna; Chino, Marco; Maglio, Ornella; Pavone, Vincenzo; Nastri, Flavia; Lombardi, Angela. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 19:10(2018), p. 2896. [10.3390/ijms19102896]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/723347
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