A novel method for the analysis of proteinaceous materials present on painted surfaces was developed by taking advantage of the adhesive ability of some fungal proteins which can form a stable and homogeneous layer on flexible transparency sheets able to capture trypsin in a fully active form. We demonstrated that the bioactive sheets wereable to efficiently digest proteins, present as such, on surfaces of painted tests and historical samples, releasing peptides that can allow an easy and confident identification of the proteinaceous binders by standard bottom-up proteomic approach. By this method there is no need: (i) to transport the artifacts and (ii) to remove, even at micro level, a sample from the object. The ingenuity of the method lies in the easily accommodated sampling coupled with a minimal invasiveness

A minimally-invasive and portable method for the identification of proteins in ancient paintings / Cicatiello, Paola; Ntasi, Georgia; Rossi, Manuela; Marino, Gennaro; Giardina, Paola; Birolo, Leila. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - 90:17(2018), pp. 10128-10133-10133. [10.1021/acs.analchem.8b01718]

A minimally-invasive and portable method for the identification of proteins in ancient paintings

Cicatiello, Paola;Rossi, Manuela;Marino, Gennaro;Giardina, Paola;Birolo, Leila
2018

Abstract

A novel method for the analysis of proteinaceous materials present on painted surfaces was developed by taking advantage of the adhesive ability of some fungal proteins which can form a stable and homogeneous layer on flexible transparency sheets able to capture trypsin in a fully active form. We demonstrated that the bioactive sheets wereable to efficiently digest proteins, present as such, on surfaces of painted tests and historical samples, releasing peptides that can allow an easy and confident identification of the proteinaceous binders by standard bottom-up proteomic approach. By this method there is no need: (i) to transport the artifacts and (ii) to remove, even at micro level, a sample from the object. The ingenuity of the method lies in the easily accommodated sampling coupled with a minimal invasiveness
2018
A minimally-invasive and portable method for the identification of proteins in ancient paintings / Cicatiello, Paola; Ntasi, Georgia; Rossi, Manuela; Marino, Gennaro; Giardina, Paola; Birolo, Leila. - In: ANALYTICAL CHEMISTRY. - ISSN 0003-2700. - 90:17(2018), pp. 10128-10133-10133. [10.1021/acs.analchem.8b01718]
File in questo prodotto:
File Dimensione Formato  
2018. Minimally Invasive and Portable Method for the Identification of protein.pdf

solo utenti autorizzati

Descrizione: 2018. Minimally Invasive and Portable Method for the Identification of protein
Tipologia: Documento in Post-print
Licenza: Accesso privato/ristretto
Dimensione 5.9 MB
Formato Adobe PDF
5.9 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/721458
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 13
social impact