A microfluidic based multiplex fluorescence signal readout system, able to fast, simply and accurately detect low concentrations of microRNA (miRNA) in small sample volumes is presented. Our ap-proach is able to simultaneously process several different miRNA types and moreover to measure their absolute amounts in a continuous microfluidic flow, without complex pre-treatments as PCR amplification steps. We additionally investigated the tendency of the utilized barcoded microgel parti-cles to be aligned, compared to rigid polystyrene particles. An improved 3D alignment has been ob-tained, according to the higher viscoelastic migration to the centreline due to the elasticity of the soft microgel particles.

Microgel-barcode readout for miRNA quantification in microfluidic flow / Rossi, D.; Dannhauser, D.; Causa, F.; Netti, P. A.. - (2016), pp. 1633-1634. (Intervento presentato al convegno 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 tenutosi a Dublin, Ireland nel 9-13 October 2016).

Microgel-barcode readout for miRNA quantification in microfluidic flow

Dannhauser D.;Causa F.;Netti P. A.
2016

Abstract

A microfluidic based multiplex fluorescence signal readout system, able to fast, simply and accurately detect low concentrations of microRNA (miRNA) in small sample volumes is presented. Our ap-proach is able to simultaneously process several different miRNA types and moreover to measure their absolute amounts in a continuous microfluidic flow, without complex pre-treatments as PCR amplification steps. We additionally investigated the tendency of the utilized barcoded microgel parti-cles to be aligned, compared to rigid polystyrene particles. An improved 3D alignment has been ob-tained, according to the higher viscoelastic migration to the centreline due to the elasticity of the soft microgel particles.
2016
978-097980649-0
Microgel-barcode readout for miRNA quantification in microfluidic flow / Rossi, D.; Dannhauser, D.; Causa, F.; Netti, P. A.. - (2016), pp. 1633-1634. (Intervento presentato al convegno 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 tenutosi a Dublin, Ireland nel 9-13 October 2016).
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/721362
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact