The interaction of the porphyrin derivative H2TCPPSpm4, having four spermines in the meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG, a sequence that show anti-HIV activity, has been investigated by means of UV, CD and PAGE studies. The results demonstrate that porphyrin substituted with positively charged side chains is capable of inducing the formation of different complexes with parallel GQs, including higherordered complexes, depending on the method used to achieve their relative stoichiometry (titration vs single addition). Indeed the CD melting experiments indicated different thermal stability fort the 1:1 H2TCPPSpm4:GQ and the 2:1 H2TCPPSpm4:GQ species obtained by titration. Interestingly also the 2:1 H2TCPPSpm4:GQ complexes obtained by titration or by direct mixing show different behavior.
Induction of G-Quadruplex Superstructures by Porphyrin Derivative Having Spermine Arms / D'Urso, Alessandro; Randazzo, Rosalba; Izzo, Valeria; Gangemi, Chiara M. A.; Romanucci, Valeria; Zarrelli, Armando; Tomaselli, Gaetano; Milardi, Danilo; Borbone, Nicola; Purrello, Roberto; Piccialli, Gennaro; DI FABIO, Giovanni; Oliviero, Giorgia. - I:(2017). (Intervento presentato al convegno II International Summer School on Natural Products “Luigi Minale” and “Ernesto Fattorusso” tenutosi a Stazione Zoologica Anton Dohrn, Facoltà di Biotecnologie dell' Università di Napoli Federico II; Napoli, Italia nel 3-7 Luglio 2017).
Induction of G-Quadruplex Superstructures by Porphyrin Derivative Having Spermine Arms
Valeria Romanucci;Armando Zarrelli;Nicola Borbone;Gennaro Piccialli;Giovanni Di Fabio;Giorgia Oliviero
2017
Abstract
The interaction of the porphyrin derivative H2TCPPSpm4, having four spermines in the meso positions, with the G-quadruplex (GQ) structure formed by the DNA aptamer TGGGAG, a sequence that show anti-HIV activity, has been investigated by means of UV, CD and PAGE studies. The results demonstrate that porphyrin substituted with positively charged side chains is capable of inducing the formation of different complexes with parallel GQs, including higherordered complexes, depending on the method used to achieve their relative stoichiometry (titration vs single addition). Indeed the CD melting experiments indicated different thermal stability fort the 1:1 H2TCPPSpm4:GQ and the 2:1 H2TCPPSpm4:GQ species obtained by titration. Interestingly also the 2:1 H2TCPPSpm4:GQ complexes obtained by titration or by direct mixing show different behavior.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
