Herein, direct determination of small RNAs is described using a functional-polymer modified genosensor. The analytical strategy adopted involves deposition by electropolymerization of biotinylated polythiophene films on the surface of miniaturized, disposable, gold screen-printed electrodes, followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes. A small RNA (miR-221) target was determined via the impedimetric measurement of the hybridization event in a label-free and PCR-free approach. Under optimized conditions, the limit of detection (LOD) was 0.7 pM miR-221 (15% RSD). The genosensor was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer cell lines, discriminating between the cancer-positive and-negative cells, without any amplification step, in less than 2 h.
Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor / Voccia, Diego; Sosnowska, Marta; Bettazzi, Francesc; Roscigno, Giuseppina; Fratini, Emiliano; De Franciscis, Vittorio; Condorelli, Gerolama; Chitta, Raghu; D'Souza, Francis; Kutner, Wlodzimierz; Palchetti, Ilaria. - In: BIOSENSORS & BIOELECTRONICS. - ISSN 0956-5663. - 87:(2017), pp. 1012-1019. [10.1016/j.bios.2016.09.058]
Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor
a Roscigno Giuseppina;Condorelli Gerolama;
2017
Abstract
Herein, direct determination of small RNAs is described using a functional-polymer modified genosensor. The analytical strategy adopted involves deposition by electropolymerization of biotinylated polythiophene films on the surface of miniaturized, disposable, gold screen-printed electrodes, followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes. A small RNA (miR-221) target was determined via the impedimetric measurement of the hybridization event in a label-free and PCR-free approach. Under optimized conditions, the limit of detection (LOD) was 0.7 pM miR-221 (15% RSD). The genosensor was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer cell lines, discriminating between the cancer-positive and-negative cells, without any amplification step, in less than 2 h.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
