ABSTRACT Objective: The link between metabolic derangement of the gut–2013liver–visceral white adipose tissue (v-WAT) axis and gut microbiota was investigated. Methods: Rats were fed a fructose-rich diet and treated with an antibiotic mix. Inflammation was measured in portal plasma, ileum, liver, and v-WAT, while insulin signalling was analysed by measuring levels of phosphorylated kinase Akt. The function and oxidative status of hepatic mitochondria and caecal microbiota composition were also evaluated. Results: Ileal inflammation, increase in plasma transaminases, plasma peroxidised lipids, portal concentrations of tumour necrosis factor alpha, lipopolysaccharide, and non-esterified fatty acids, were induced by fructose and were reversed by antibiotic. The increased hepatic ceramide content, inflammation and decreased insulin signaling in liver and v-WAT induced by fructose was reversed by antibiotic. Antibiotic also blunted the increase in hepatic mitochondrial efficiency and oxidative damage of rats fed fructose-rich diet. Three genera, Coprococcus, Ruminococcus, and Clostridium, significantly increased, while the Clostridiaceae family significantly decreased in rats fed a fructose-rich diet, and antibiotic abolished these variations Conclusions: When gut microbiota modulation by fructose is prevented by antibiotic, inflamma- tory flow from the gut to the liver and v-WAT are reversed.

Dietary fructose causes defective insulin signaling and ceramide accumulation that can be reversed by gut microbiota modulation / Crescenzo, R; Mazzoli, A; CRESCENZO AND MAZZOLI: FIRST, Coauthorship; Di Luccia, B; Bianco, F; Cancelliere, R; Cigliano, L; Liverini, G; Baccigalupi, L; Iossa, S. - In: FOOD & NUTRITION RESEARCH. - ISSN 1654-661X. - 61:1(2017), pp. 1-14. [10.1080/16546628.2017.1331657]

Dietary fructose causes defective insulin signaling and ceramide accumulation that can be reversed by gut microbiota modulation.

Crescenzo, R;Mazzoli, A;Di Luccia, B;Cancelliere, R;Cigliano, L;Liverini, G;Baccigalupi, L;Iossa, S
2017

Abstract

ABSTRACT Objective: The link between metabolic derangement of the gut–2013liver–visceral white adipose tissue (v-WAT) axis and gut microbiota was investigated. Methods: Rats were fed a fructose-rich diet and treated with an antibiotic mix. Inflammation was measured in portal plasma, ileum, liver, and v-WAT, while insulin signalling was analysed by measuring levels of phosphorylated kinase Akt. The function and oxidative status of hepatic mitochondria and caecal microbiota composition were also evaluated. Results: Ileal inflammation, increase in plasma transaminases, plasma peroxidised lipids, portal concentrations of tumour necrosis factor alpha, lipopolysaccharide, and non-esterified fatty acids, were induced by fructose and were reversed by antibiotic. The increased hepatic ceramide content, inflammation and decreased insulin signaling in liver and v-WAT induced by fructose was reversed by antibiotic. Antibiotic also blunted the increase in hepatic mitochondrial efficiency and oxidative damage of rats fed fructose-rich diet. Three genera, Coprococcus, Ruminococcus, and Clostridium, significantly increased, while the Clostridiaceae family significantly decreased in rats fed a fructose-rich diet, and antibiotic abolished these variations Conclusions: When gut microbiota modulation by fructose is prevented by antibiotic, inflamma- tory flow from the gut to the liver and v-WAT are reversed.
2017
Dietary fructose causes defective insulin signaling and ceramide accumulation that can be reversed by gut microbiota modulation / Crescenzo, R; Mazzoli, A; CRESCENZO AND MAZZOLI: FIRST, Coauthorship; Di Luccia, B; Bianco, F; Cancelliere, R; Cigliano, L; Liverini, G; Baccigalupi, L; Iossa, S. - In: FOOD & NUTRITION RESEARCH. - ISSN 1654-661X. - 61:1(2017), pp. 1-14. [10.1080/16546628.2017.1331657]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/700430
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