Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.
Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea / Salbitani, Giovanna; Bottone, Claudia; Carfagna, Simona. - In: BIO-PROTOCOL. - ISSN 2331-8325. - 7:13(2017). [10.21769/BioProtoc.2372]
Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
Salbitani, Giovanna;Bottone, Claudia;Carfagna, Simona
2017
Abstract
Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved spectrophotometric method to estimate the content of reduced (GSH) and oxidized (GSSG) forms of glutathione in the extremophilic microalga Galdieria phlegrea.| File | Dimensione | Formato | |
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