A cost-effective immunosensor for the detection and isolation of dental pulp stemcells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specificmonoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCMsurface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with amixed cell population, was analyzed; the results indicated that the developed QCMimmunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell fromsampleswith high complexity, being simple, selective, and providing for more convenient and time-saving operations. 

A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction / Maglio, Ornella; Costanzo, Salvatore; Cercola, Rosaria; Zambrano, Gerardo; Mauro, Marco; Battaglia, Raffaele; Ferrini, Gianluca; Nastri, Flavia; Pavone, Vincenzo; Lombardi, Angelina. - In: SENSORS. - ISSN 1424-8220. - 17:12(2017), p. 2747. [10.3390/s17122747]

A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

Ornella Maglio;Gerardo Zambrano;Marco Mauro;Raffaele Battaglia;Gianluca Ferrini;Flavia Nastri;Vincenzo Pavone;Angelina Lombardi
2017

Abstract

A cost-effective immunosensor for the detection and isolation of dental pulp stemcells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specificmonoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCMsurface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with amixed cell population, was analyzed; the results indicated that the developed QCMimmunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell fromsampleswith high complexity, being simple, selective, and providing for more convenient and time-saving operations. 
2017
A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction / Maglio, Ornella; Costanzo, Salvatore; Cercola, Rosaria; Zambrano, Gerardo; Mauro, Marco; Battaglia, Raffaele; Ferrini, Gianluca; Nastri, Flavia; Pavone, Vincenzo; Lombardi, Angelina. - In: SENSORS. - ISSN 1424-8220. - 17:12(2017), p. 2747. [10.3390/s17122747]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/698749
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