In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such as K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.
Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies / Palmirotta, Raffaele; De Marchis, Maria Laura; Ludovici, Giorgia; Ferroni, Patrizia; Abete, Pasquale; Guadagni, Fiorella; Della-morte, David. - In: BIOCHEMISTRY. - ISSN 0006-2960. - 1175:(2014), pp. 45-65. [10.1007/978-1-4939-0956-8_4]
Diagnostic procedures for paraffi n-embedded tissues analysis in pharmacogenomic studies
Abete, Pasquale;
2014
Abstract
In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such as K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffi n-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffi n-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.| File | Dimensione | Formato | |
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