gH625 constitutes a promising delivery vehicle for the transport of therapeutic biomacromolecules across membrane barriers. We report an application of multivalency to create a complex nanosystem for delivery and to elucidate the mechanism of peptide-lipid bilayer interactions. Multivalency may offer a route to enhance gH625 cellular uptake as demonstrated by results obtained on dimers of gH625 by fluorescence spectroscopy, circular dichroism, and surface plasmon resonance. Moreover, using both phase contrast and light sheet fluorescence microscopy we were able to characterize and visualize for the first time the fusion of giant unilamellar vesicles caused by a membranotropic peptide.

Dimerization in tailoring uptake efficacy of the HSV-1 derived membranotropic peptide gH625 / Falanga, Annarita; Valiante, Salvatore; Galdiero, Emilia; Franci, Gianluigi; Scudiero, Olga; Morelli, Giancarlo; Galdiero, Stefania. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017), pp. 9434-9453. [10.1038/s41598-017-09001-x]

Dimerization in tailoring uptake efficacy of the HSV-1 derived membranotropic peptide gH625

FALANGA, ANNARITA
Primo
;
VALIANTE, Salvatore;GALDIERO, EMILIA;SCUDIERO, OLGA;MORELLI, GIANCARLO;GALDIERO, STEFANIA
Ultimo
Writing – Review & Editing
2017

Abstract

gH625 constitutes a promising delivery vehicle for the transport of therapeutic biomacromolecules across membrane barriers. We report an application of multivalency to create a complex nanosystem for delivery and to elucidate the mechanism of peptide-lipid bilayer interactions. Multivalency may offer a route to enhance gH625 cellular uptake as demonstrated by results obtained on dimers of gH625 by fluorescence spectroscopy, circular dichroism, and surface plasmon resonance. Moreover, using both phase contrast and light sheet fluorescence microscopy we were able to characterize and visualize for the first time the fusion of giant unilamellar vesicles caused by a membranotropic peptide.
2017
Dimerization in tailoring uptake efficacy of the HSV-1 derived membranotropic peptide gH625 / Falanga, Annarita; Valiante, Salvatore; Galdiero, Emilia; Franci, Gianluigi; Scudiero, Olga; Morelli, Giancarlo; Galdiero, Stefania. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017), pp. 9434-9453. [10.1038/s41598-017-09001-x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/683227
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