Herein we present a textile wearable electrochemical transistor by functionalizing a single cotton yarn with semiconducting polymer. The organic electrochemical transistor (OECT), which is low cost and completely integrated e-textile, is decorated by adsorption of the fungal laccase POXA1b, and is used as biosensor for the direct detection of Tyrosine (L-Tyr) without the use of electron mediators. The detection of Tyr in real-case scenario such as human physiological fluids would own a paramount importance in noninvasive analysis of the patient's condition, monitoring and preventing several pathologies. To assess the reaction progression, the redox process is studied by UV–visible absorption with test reference molecule of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS): the results confirmed that the oxidation reaction is driven by the presence of laccase enzyme and direct electron transfer occurred. The modulation of the signal response and the kinetic of the signal is used to detect Tyr molecule in aqueous solution and the role of the enzyme adsorption on the textile is analyzed. A kinetic analysis of the characteristic modulation times of the sensing curves, confirm the sensing properties of the textile device. The textile-based biosensor is demonstrated to monitor human health biomarkers through wearable applications in a non-invasive way, finding potential application in sport, healthcare and working safety.

Enzymatic sensing with laccase-functionalized textile organic biosensors / Battista, Edmondo; Lettera, Vincenzo; Villani, Marco; Calestani, Davide; Gentile, Francesco; Netti, PAOLO ANTONIO; Iannotta, Salvatore; Zappettini, Andrea; Coppedè, Nicola. - In: ORGANIC ELECTRONICS. - ISSN 1566-1199. - 40:(2017), pp. 51-57. [10.1016/j.orgel.2016.10.037]

Enzymatic sensing with laccase-functionalized textile organic biosensors

BATTISTA, EDMONDO;LETTERA, VINCENZO;GENTILE, Francesco;NETTI, PAOLO ANTONIO;
2017

Abstract

Herein we present a textile wearable electrochemical transistor by functionalizing a single cotton yarn with semiconducting polymer. The organic electrochemical transistor (OECT), which is low cost and completely integrated e-textile, is decorated by adsorption of the fungal laccase POXA1b, and is used as biosensor for the direct detection of Tyrosine (L-Tyr) without the use of electron mediators. The detection of Tyr in real-case scenario such as human physiological fluids would own a paramount importance in noninvasive analysis of the patient's condition, monitoring and preventing several pathologies. To assess the reaction progression, the redox process is studied by UV–visible absorption with test reference molecule of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS): the results confirmed that the oxidation reaction is driven by the presence of laccase enzyme and direct electron transfer occurred. The modulation of the signal response and the kinetic of the signal is used to detect Tyr molecule in aqueous solution and the role of the enzyme adsorption on the textile is analyzed. A kinetic analysis of the characteristic modulation times of the sensing curves, confirm the sensing properties of the textile device. The textile-based biosensor is demonstrated to monitor human health biomarkers through wearable applications in a non-invasive way, finding potential application in sport, healthcare and working safety.
2017
Enzymatic sensing with laccase-functionalized textile organic biosensors / Battista, Edmondo; Lettera, Vincenzo; Villani, Marco; Calestani, Davide; Gentile, Francesco; Netti, PAOLO ANTONIO; Iannotta, Salvatore; Zappettini, Andrea; Coppedè, Nicola. - In: ORGANIC ELECTRONICS. - ISSN 1566-1199. - 40:(2017), pp. 51-57. [10.1016/j.orgel.2016.10.037]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/679152
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 51
  • ???jsp.display-item.citation.isi??? 45
social impact