The importance of carbohydrates in a variety of biological functions is the reason that interest has recently increased in these compounds as possible components of therapeutic agents. Thus, the need for a technique allowing the easy synthesis of carbohydrates and glucoconjugates is an emerging challenge for chemists and biologists involved in this field. At present, enzymatic synthesis has resulted in the most promising approach for the production of complex oligosaccharides. In this respect, the enzymological charac- teristics of the catalysts, in term of regioselectivity, substrate specificity, and operational stability, are of fundamental importance to improve the yields of the process and to widen the repertoire of the available products. Here, two methods of oligosaccharide synthesis performed by a glyco- synthase and by an α-xylosidase from the hyperthermo- philic archaeon Sulfolobus solfataricus are briefly reviewed. The approaches used and the biodiversity of the catalysts together are key features for their possible utilization in the synthesis of oligosaccharides
Enzymatic synthesis and hydrolysis of xylogluco-oligosaccharides using the first archaeal α-xylosidase from Sulfolobus solfataricus / Trincone, Antonio; Cobucci Ponzano, Beatrice; DI LAURO, Barbara; Rossi, Mose'; Mitsuishi, Yasushi; Moracci, Marco. - In: EXTREMOPHILES. - ISSN 1431-0651. - 5:4(2001), pp. 277-282. [10.1007/s007920100204]
Enzymatic synthesis and hydrolysis of xylogluco-oligosaccharides using the first archaeal α-xylosidase from Sulfolobus solfataricus
DI LAURO, BARBARA;ROSSI, MOSE';MORACCI, Marco
2001
Abstract
The importance of carbohydrates in a variety of biological functions is the reason that interest has recently increased in these compounds as possible components of therapeutic agents. Thus, the need for a technique allowing the easy synthesis of carbohydrates and glucoconjugates is an emerging challenge for chemists and biologists involved in this field. At present, enzymatic synthesis has resulted in the most promising approach for the production of complex oligosaccharides. In this respect, the enzymological charac- teristics of the catalysts, in term of regioselectivity, substrate specificity, and operational stability, are of fundamental importance to improve the yields of the process and to widen the repertoire of the available products. Here, two methods of oligosaccharide synthesis performed by a glyco- synthase and by an α-xylosidase from the hyperthermo- philic archaeon Sulfolobus solfataricus are briefly reviewed. The approaches used and the biodiversity of the catalysts together are key features for their possible utilization in the synthesis of oligosaccharidesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.