Correct spatial and temporal control of cell proliferation is of fundamental importance for tissue homeostasis. Its deregulation has been associated with several pathological conditions. In common with almost every aspect of plant and animal biology, cell proliferation is dominated by day-night rhythms generated by the circadian clock. However, our understanding of the crosstalk between the core clock and cell cycle control mechanisms remains incomplete. In this study, using zebrafish as a vertebrate model system, we show that the nuclear localization of the Y-box binding protein 1 (YB-1), a regulator of cyclin expression and a hallmark of certain cancers, is robustly regulated by the circadian clock. We implicate clock-controlled changes in YB-1 SUMOylation as one of the mechanisms regulating its periodic nuclear entry at the beginning of the light phase. Furthermore, we demonstrate that YB-1 nuclear protein is able to downregulate cyclin A2 mRNA expression in zebrafish via its direct interaction with the cyclin A2 promoter. Thus, by acting as a direct target of cyclic posttranslational regulatory mechanisms, YB-1 serves as one bridge between the circadian clock and its cell cycle control.

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation / Pagano, Cristina; di Martino, Orsola; Ruggiero, Gennaro; Maria Guarino, Andrea; Mueller, Nathalie; Siauciunaite, Rima; Reischl, Markus; Simon Foulkes, Nicholas; Vallone, Daniela; Calabro', Viola. - In: ONCOTARGET. - ISSN 1949-2553. - 8:4(2017), pp. 6193-6205. [10.18632/oncotarget.14051]

The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation

Pagano, Cristina
Investigation
;
di Martino, Orsola
Investigation
;
Maria Guarino, Andrea
Investigation
;
Calabro', Viola
2017

Abstract

Correct spatial and temporal control of cell proliferation is of fundamental importance for tissue homeostasis. Its deregulation has been associated with several pathological conditions. In common with almost every aspect of plant and animal biology, cell proliferation is dominated by day-night rhythms generated by the circadian clock. However, our understanding of the crosstalk between the core clock and cell cycle control mechanisms remains incomplete. In this study, using zebrafish as a vertebrate model system, we show that the nuclear localization of the Y-box binding protein 1 (YB-1), a regulator of cyclin expression and a hallmark of certain cancers, is robustly regulated by the circadian clock. We implicate clock-controlled changes in YB-1 SUMOylation as one of the mechanisms regulating its periodic nuclear entry at the beginning of the light phase. Furthermore, we demonstrate that YB-1 nuclear protein is able to downregulate cyclin A2 mRNA expression in zebrafish via its direct interaction with the cyclin A2 promoter. Thus, by acting as a direct target of cyclic posttranslational regulatory mechanisms, YB-1 serves as one bridge between the circadian clock and its cell cycle control.
2017
The tumor-associated YB-1 protein: new player in the circadian control of cell proliferation / Pagano, Cristina; di Martino, Orsola; Ruggiero, Gennaro; Maria Guarino, Andrea; Mueller, Nathalie; Siauciunaite, Rima; Reischl, Markus; Simon Foulkes, Nicholas; Vallone, Daniela; Calabro', Viola. - In: ONCOTARGET. - ISSN 1949-2553. - 8:4(2017), pp. 6193-6205. [10.18632/oncotarget.14051]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/671907
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