Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered amodel to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34Hproduces two different extracellular polysaccharides, a capsular polysaccharide and amedium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and twodimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6- trideoxy-glucose (bacillosamine), and 2-acetamido-2- deoxyglucuronic acid with the following structure: ?4)-b-D-GlcpNAcA-(1 ?3)-b-D-QuipNAc4NAc- (1 ?3)-b-D-GalpNAc-(1 ?.The 3Dmodel, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 C, this molecule displays only a low ice recrystallization inhibition activity

Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H / Casillo, Angela; Ståhle, Jonas; Parrilli, Ermenegilda; Sannino, Filomena; Mitchell, Daniel E.; Pieretti, Giuseppina; Gibson, Matthew I.; Marino, Gennaro; Lanzetta, Rosa; Parrilli, Michelangelo; Widmalm, Göran; Tutino, MARIA LUISA; Corsaro, MARIA MICHELA. - In: ANTONIE VAN LEEUWENHOEK. - ISSN 0003-6072. - 110:(2017), pp. 1377-1387. [10.1007/s10482-017-0834-6]

Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H

Casillo, Angela;PARRILLI, ERMENEGILDA;Sannino, Filomena;Pieretti, Giuseppina;Marino, Gennaro;LANZETTA, ROSA;PARRILLI, MICHELANGELO;TUTINO, MARIA LUISA;CORSARO, MARIA MICHELA
2017

Abstract

Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered amodel to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34Hproduces two different extracellular polysaccharides, a capsular polysaccharide and amedium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and twodimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6- trideoxy-glucose (bacillosamine), and 2-acetamido-2- deoxyglucuronic acid with the following structure: ?4)-b-D-GlcpNAcA-(1 ?3)-b-D-QuipNAc4NAc- (1 ?3)-b-D-GalpNAc-(1 ?.The 3Dmodel, generated in accordance with 1H,1H-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 C, this molecule displays only a low ice recrystallization inhibition activity
2017
Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H / Casillo, Angela; Ståhle, Jonas; Parrilli, Ermenegilda; Sannino, Filomena; Mitchell, Daniel E.; Pieretti, Giuseppina; Gibson, Matthew I.; Marino, Gennaro; Lanzetta, Rosa; Parrilli, Michelangelo; Widmalm, Göran; Tutino, MARIA LUISA; Corsaro, MARIA MICHELA. - In: ANTONIE VAN LEEUWENHOEK. - ISSN 0003-6072. - 110:(2017), pp. 1377-1387. [10.1007/s10482-017-0834-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/663533
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