To date, no studies have explored the effect of abnormal cerebral venous circulation on brain disorders, whereas many studies have investigated neurodegenerative brain anomalies associated with arterial diseases. The aim of our study was to demonstrate the feasibility of different surgical techniques to induce venous obstruction of cerebral brain drainage. Six C57/black mice underwent bilateral occlusion of the external jugular vein (group EJV), six underwent bilateral occlusion of the internal jugular vein (group IJV); and six underwent bilateral occlusion of both the EJV and the IJV (group EJV/IJV). Within each group, the interruption of blood flow was obtained via monopolar electro-coagulation (ME) in three mice and via surgical ligation (SL) in the remaining three mice. A “sham group” of two mice was used as the control. High-frequency ultrasound (HFUS) was used to detect the absence of blood flow in the examined vessel. The ME procedure led to successful results in 2/9 (22%) mice, one in the EJV group, one in the EJV/IJV group, and zero in the IJV group, and 4/18 (22%) when considering individual veins (i.e., total number of EJVs and IJVs occluded). The SL procedure was successful in 2/3 (67%) mice in the EJV group, in 3/3 (100%) mice in the IJV and in 3/4 (75%) mice in the EJV/IJV group. Therefore, the overall success rate was 8/10 (80%) when considering mice, and 20/26 (77%) when considering individual veins. The monopolar electro-coagulation method exhibited a high mortality due to cardiorespiratory arrest, while the results of the bilateral surgical ligation of EJVs and IJVs show that it is technically feasible and safe.

Feasibility and safety of two surgical techniques for the development of an animal model of jugular vein occlusion / Auletta, L.; Greco, A.; Albanese, S.; Meomartino, L.; Salvatore, M.; Mancini., M.. - In: EXPERIMENTAL BIOLOGY AND MEDICINE. - ISSN 1535-3702. - (2017), pp. 22-28. [10.1177/1535370216657446]

Feasibility and safety of two surgical techniques for the development of an animal model of jugular vein occlusion.

A. Greco;L. Meomartino;
2017

Abstract

To date, no studies have explored the effect of abnormal cerebral venous circulation on brain disorders, whereas many studies have investigated neurodegenerative brain anomalies associated with arterial diseases. The aim of our study was to demonstrate the feasibility of different surgical techniques to induce venous obstruction of cerebral brain drainage. Six C57/black mice underwent bilateral occlusion of the external jugular vein (group EJV), six underwent bilateral occlusion of the internal jugular vein (group IJV); and six underwent bilateral occlusion of both the EJV and the IJV (group EJV/IJV). Within each group, the interruption of blood flow was obtained via monopolar electro-coagulation (ME) in three mice and via surgical ligation (SL) in the remaining three mice. A “sham group” of two mice was used as the control. High-frequency ultrasound (HFUS) was used to detect the absence of blood flow in the examined vessel. The ME procedure led to successful results in 2/9 (22%) mice, one in the EJV group, one in the EJV/IJV group, and zero in the IJV group, and 4/18 (22%) when considering individual veins (i.e., total number of EJVs and IJVs occluded). The SL procedure was successful in 2/3 (67%) mice in the EJV group, in 3/3 (100%) mice in the IJV and in 3/4 (75%) mice in the EJV/IJV group. Therefore, the overall success rate was 8/10 (80%) when considering mice, and 20/26 (77%) when considering individual veins. The monopolar electro-coagulation method exhibited a high mortality due to cardiorespiratory arrest, while the results of the bilateral surgical ligation of EJVs and IJVs show that it is technically feasible and safe.
2017
Feasibility and safety of two surgical techniques for the development of an animal model of jugular vein occlusion / Auletta, L.; Greco, A.; Albanese, S.; Meomartino, L.; Salvatore, M.; Mancini., M.. - In: EXPERIMENTAL BIOLOGY AND MEDICINE. - ISSN 1535-3702. - (2017), pp. 22-28. [10.1177/1535370216657446]
File in questo prodotto:
File Dimensione Formato  
Feasibility Safety 2 Surgical Techniques Development Animal Model JV Occlusion - Auletta.pdf

non disponibili

Descrizione: articolo post print
Tipologia: Documento in Post-print
Licenza: Accesso privato/ristretto
Dimensione 581.91 kB
Formato Adobe PDF
581.91 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/659625
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact