Microbialbiofilmshavegreatnegativeimpactsontheworld’seconomyandposeseriousproblemstoindustry,publichealthandmedicine.Theinterestinthedevelopmentofnewapproachesforthepreventionandtreatmentofbacterialadhesionandbiofilmformationhasincreased.Since,bacterialpathogenslivinginbiofilminducepersistentchronicinfectionsduetotheresistancetoantibioticsandhostimmunesystem.Aviableapproachshouldtargetadhesivepropertieswithoutaffectingbacterialvitalityinordertoavoidtheappearanceofresistantmutants.Manybacteriasecreteanti-biofilmmoleculesthatfunctioninregulatingbiofilmarchitectureormediatingthereleaseofcellsfromitduringthedispersalstageofbiofilmlifecycle.Cold-adaptedmarinebacteriarepresentanuntappedreservoirofbiodiversityabletosynthesizeabroadrangeofbioactivecompounds,includinganti-biofilmmolecules.Theanti-biofilmactivityofcell-freesupernatantsderivedfromsessileandplanktonicculturesofcold-adaptedbacteriabelongingtoPseudoalteromonas,Psychrobacter,andPsychromonasspeciesweretestedagainstStaphylococcusaureus,Staphylococcusepidermidis,andPseudomonasaeruginosastrains.Reportedresultsdemonstratethatwehaveselectedsupernatants,fromcold-adaptedmarinebacteria,containingnon-biocidalagentsabletodestabilizebiofilmmatrixofalltestedpathogenswithoutkillingcells.Apreliminaryphysico-chemicalcharacterizationofsupernatantswasalsoperformed,andtheseanalyseshighlightedthepresenceofmoleculesofdifferentnaturethatactbyinhibitingbiofilmformation.Someofthemarealsoabletoimpairtheinitialattachmentofthebacterialcellstothesurface,thuslikelycontainingmoleculesactingasanti-biofilmsurfactantmolecules.Thedescribedabilityofcold-adaptedbacteriatoproduceeffectiveanti-biofilmmoleculespavesthewaytofurthercharacterizationofthemostpromisingmoleculesandtotesttheiruseincombinationwithconventionalantibiotics.
Anti-biofilm activities from marine cold adapted bacteria against staphylococci and Pseudomonas aeruginosa / Papa, Rosanna; Selan, Laura; Parrilli, Ermenegilda; Tilotta, Marco; Sannino, Filomena; Feller, Georges; Tutino, MARIA LUISA; Artini, Marco. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 6:DEC-Article number 01333(2015), pp. 1-10. [10.3389/fmicb.2015.01333]
Anti-biofilm activities from marine cold adapted bacteria against staphylococci and Pseudomonas aeruginosa
PARRILLI, ERMENEGILDA;TUTINO, MARIA LUISA;
2015
Abstract
Microbialbiofilmshavegreatnegativeimpactsontheworld’seconomyandposeseriousproblemstoindustry,publichealthandmedicine.Theinterestinthedevelopmentofnewapproachesforthepreventionandtreatmentofbacterialadhesionandbiofilmformationhasincreased.Since,bacterialpathogenslivinginbiofilminducepersistentchronicinfectionsduetotheresistancetoantibioticsandhostimmunesystem.Aviableapproachshouldtargetadhesivepropertieswithoutaffectingbacterialvitalityinordertoavoidtheappearanceofresistantmutants.Manybacteriasecreteanti-biofilmmoleculesthatfunctioninregulatingbiofilmarchitectureormediatingthereleaseofcellsfromitduringthedispersalstageofbiofilmlifecycle.Cold-adaptedmarinebacteriarepresentanuntappedreservoirofbiodiversityabletosynthesizeabroadrangeofbioactivecompounds,includinganti-biofilmmolecules.Theanti-biofilmactivityofcell-freesupernatantsderivedfromsessileandplanktonicculturesofcold-adaptedbacteriabelongingtoPseudoalteromonas,Psychrobacter,andPsychromonasspeciesweretestedagainstStaphylococcusaureus,Staphylococcusepidermidis,andPseudomonasaeruginosastrains.Reportedresultsdemonstratethatwehaveselectedsupernatants,fromcold-adaptedmarinebacteria,containingnon-biocidalagentsabletodestabilizebiofilmmatrixofalltestedpathogenswithoutkillingcells.Apreliminaryphysico-chemicalcharacterizationofsupernatantswasalsoperformed,andtheseanalyseshighlightedthepresenceofmoleculesofdifferentnaturethatactbyinhibitingbiofilmformation.Someofthemarealsoabletoimpairtheinitialattachmentofthebacterialcellstothesurface,thuslikelycontainingmoleculesactingasanti-biofilmsurfactantmolecules.Thedescribedabilityofcold-adaptedbacteriatoproduceeffectiveanti-biofilmmoleculespavesthewaytofurthercharacterizationofthemostpromisingmoleculesandtotesttheiruseincombinationwithconventionalantibiotics.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
