In the last decade, DNA duplex and G-quadruplex motifs have been investigated for their potential applications in nanotechnology. Recently, G-quadruplex-duplex hybrids, generated from the juxtaposition of these two structural elements, have been considered as DNA nanostructures for nanotechnological applications to take advantage of both duplex and G-quadruplex peculiarities. The junction between the two structural motifs can play an important role both for the structure and stability of these hybrids. Here, we analyze the thermodynamics of a number of G-quadruplex-duplex hybrids differing in the bases composition in proximity of the junction. Differential scanning calorimetry, circular dichroism, and gel electrophoresis methodologies were employed to highlight differences in their stability.

How are thermodynamically stable G-quadruplex-duplex hybrids?

FOTTICCHIA, IOLANDA;AMATO, JUSSARA;PAGANO, BRUNO;NOVELLINO, ETTORE;PETRACCONE, LUIGI;GIANCOLA, CONCETTA
2015

Abstract

In the last decade, DNA duplex and G-quadruplex motifs have been investigated for their potential applications in nanotechnology. Recently, G-quadruplex-duplex hybrids, generated from the juxtaposition of these two structural elements, have been considered as DNA nanostructures for nanotechnological applications to take advantage of both duplex and G-quadruplex peculiarities. The junction between the two structural motifs can play an important role both for the structure and stability of these hybrids. Here, we analyze the thermodynamics of a number of G-quadruplex-duplex hybrids differing in the bases composition in proximity of the junction. Differential scanning calorimetry, circular dichroism, and gel electrophoresis methodologies were employed to highlight differences in their stability.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/647568
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 5
social impact