Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique which uses a pulsed laser beam impinging, inside a high vacuum chamber, on a frozen target containing the guest molecules in a volatile matrix to induce fast “evaporation” of the matrix, and ejection of the guest molecules. Lipase, an enzyme acting as a catalyst in hydrolysis of lipids, is widely used in biosensors for detection of triglycerides in blood serum. A key action to this purpose is lipase immobilization on a substrate. In a recent paper, we have shown that MAPLE technique is able to deposit lipase on a substrate in an active form. Here we show that addition to the guest/matrix target of a small amount of m-DOPA (3-(3,4-dihydroxyphenyl)-2-methyl-l-alanine) in order to improve adhesion and protect lipase secondary structure, also allows the lowering the laser pulse energy required for matrix evaporation and therefore the risk of damaging the enzyme.

m-DOPA addition in MAPLE immobilization of lipase for biosensor applications / Califano, Valeria; Ausanio, Giovanni; Bloisi, Francesco; Aronne, Antonio; Vicari, LUCIANO ROSARIO MARIA; Nasti, Libera. - In: SENSING AND BIO-SENSING RESEARCH. - ISSN 2214-1804. - 6:(2015), pp. 103-108. [10.1016/j.sbsr.2015.07.007]

m-DOPA addition in MAPLE immobilization of lipase for biosensor applications

AUSANIO, GIOVANNI;BLOISI, FRANCESCO;ARONNE, ANTONIO;VICARI, LUCIANO ROSARIO MARIA;NASTI, Libera
2015

Abstract

Matrix Assisted Pulsed Laser Evaporation (MAPLE) is a thin film deposition technique which uses a pulsed laser beam impinging, inside a high vacuum chamber, on a frozen target containing the guest molecules in a volatile matrix to induce fast “evaporation” of the matrix, and ejection of the guest molecules. Lipase, an enzyme acting as a catalyst in hydrolysis of lipids, is widely used in biosensors for detection of triglycerides in blood serum. A key action to this purpose is lipase immobilization on a substrate. In a recent paper, we have shown that MAPLE technique is able to deposit lipase on a substrate in an active form. Here we show that addition to the guest/matrix target of a small amount of m-DOPA (3-(3,4-dihydroxyphenyl)-2-methyl-l-alanine) in order to improve adhesion and protect lipase secondary structure, also allows the lowering the laser pulse energy required for matrix evaporation and therefore the risk of damaging the enzyme.
2015
m-DOPA addition in MAPLE immobilization of lipase for biosensor applications / Califano, Valeria; Ausanio, Giovanni; Bloisi, Francesco; Aronne, Antonio; Vicari, LUCIANO ROSARIO MARIA; Nasti, Libera. - In: SENSING AND BIO-SENSING RESEARCH. - ISSN 2214-1804. - 6:(2015), pp. 103-108. [10.1016/j.sbsr.2015.07.007]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/647489
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