Nowadays, microfluidic channels of a few tens of micrometers are required and widely used in many fields, especially for surface-processing applications and miniaturization of biological assays. Herein, we selected micromilling as a low-cost technology and proposed an approach capable of overcoming its limitations; in fact, microstructures below 20-30 m in depth are difficult to obtain, and the manufacturing error is rather high, as it is inversely proportional to the depth. Indeed, the proposed method uses a confined dehydration process of a patterned gelatin substrate fabricated via replica molding onto a micromilled poly(methyl methacrylate) substrate to produce a gelatin master with demonstrated final micrometric features down to 3 m for the channel depth and, in specific configurations, down to 5 m for the channel width. Finally, we demonstrated the ability to flux liquids in miniaturized microfluidic devices and fabricated and tested - as an example - micrometric microstructures arrays connected via microchannels for biological assays

Confined Gelatin Dehydration as a Viable Route to Go beyond Micromilling Resolution and Miniaturize Biological Assays / Vecchione, Raffaele; Pitingolo, Gabriele; Falanga, ANDREA PATRIZIA; Guarnieri, Daniela; Netti, PAOLO ANTONIO. - In: ACS APPLIED MATERIALS & INTERFACES. - ISSN 1944-8244. - 8:19(2016), pp. 12075-12081. [10.1021/acsami.6b04128]

Confined Gelatin Dehydration as a Viable Route to Go beyond Micromilling Resolution and Miniaturize Biological Assays

FALANGA, ANDREA PATRIZIA;GUARNIERI, DANIELA;NETTI, PAOLO ANTONIO
2016

Abstract

Nowadays, microfluidic channels of a few tens of micrometers are required and widely used in many fields, especially for surface-processing applications and miniaturization of biological assays. Herein, we selected micromilling as a low-cost technology and proposed an approach capable of overcoming its limitations; in fact, microstructures below 20-30 m in depth are difficult to obtain, and the manufacturing error is rather high, as it is inversely proportional to the depth. Indeed, the proposed method uses a confined dehydration process of a patterned gelatin substrate fabricated via replica molding onto a micromilled poly(methyl methacrylate) substrate to produce a gelatin master with demonstrated final micrometric features down to 3 m for the channel depth and, in specific configurations, down to 5 m for the channel width. Finally, we demonstrated the ability to flux liquids in miniaturized microfluidic devices and fabricated and tested - as an example - micrometric microstructures arrays connected via microchannels for biological assays
2016
Confined Gelatin Dehydration as a Viable Route to Go beyond Micromilling Resolution and Miniaturize Biological Assays / Vecchione, Raffaele; Pitingolo, Gabriele; Falanga, ANDREA PATRIZIA; Guarnieri, Daniela; Netti, PAOLO ANTONIO. - In: ACS APPLIED MATERIALS & INTERFACES. - ISSN 1944-8244. - 8:19(2016), pp. 12075-12081. [10.1021/acsami.6b04128]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/642791
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 6
social impact