We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy / Merola, F.; Miccio, L.; Memmolo, P.; Di Caprio, G.; Coppola, G.; Netti, PAOLO ANTONIO; Ferraro, P.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - 8947:(2014), p. 89471A. (Intervento presentato al convegno Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII tenutosi a San Francisco, CA, usa nel 2014) [10.1117/12.2039423].

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

NETTI, PAOLO ANTONIO;
2014

Abstract

We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).
2014
9780819498601
9780819498601
3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy / Merola, F.; Miccio, L.; Memmolo, P.; Di Caprio, G.; Coppola, G.; Netti, PAOLO ANTONIO; Ferraro, P.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - 8947:(2014), p. 89471A. (Intervento presentato al convegno Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII tenutosi a San Francisco, CA, usa nel 2014) [10.1117/12.2039423].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/625186
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 0
social impact