The synthesis and a preliminary evaluation of the pairing properties of ribo-cyclohexanyl nucleic acids (r-CNA) is herein reported. Incorporation of a single r-CNA nucleotide into natural duplexes did not enhance their stability, while a very high pairing selectivity for RNA was found. As deduced by comparative analysis of Tm and NMR data, a relationship between pairing selectivity and conformational preferences of the “sugar” moiety of r-CNA (and more generally of six-membered nucleic acids) was suggested.

Oligonucleotides containing a ribo-configured cyclohexanyl nucleoside: probing the role of sugar conformation in base pairing selectivity

PAOLELLA, CONCETTA;D'ALONZO, DANIELE;DI FABIO, GIOVANNI;PALUMBO, GIOVANNI;GUARAGNA, ANNALISA
2015

Abstract

The synthesis and a preliminary evaluation of the pairing properties of ribo-cyclohexanyl nucleic acids (r-CNA) is herein reported. Incorporation of a single r-CNA nucleotide into natural duplexes did not enhance their stability, while a very high pairing selectivity for RNA was found. As deduced by comparative analysis of Tm and NMR data, a relationship between pairing selectivity and conformational preferences of the “sugar” moiety of r-CNA (and more generally of six-membered nucleic acids) was suggested.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/625018
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